TY - JOUR
T1 - Hematopoietic activity in putative mouse primordial germ cell populations
AU - Scaldaferri, Maria Lucia
AU - Klinger, Francesca Gioia
AU - Farini, Donatella
AU - Di Carlo, Anna
AU - Carsetti, Rita
AU - Giorda, Ezio
AU - De Felici, Massimo
PY - 2015/5/1
Y1 - 2015/5/1
N2 - In the present paper, starting from the observation of heterogeneous expression of the GOF-18δPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFPweak CD-34weak/high cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.
AB - In the present paper, starting from the observation of heterogeneous expression of the GOF-18δPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFPweak CD-34weak/high cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.
KW - Allantois
KW - Germline
KW - Hematopoiesis
KW - Oct3/4
KW - Primordial germ cells
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U2 - 10.1016/j.mod.2015.02.002
DO - 10.1016/j.mod.2015.02.002
M3 - Article
C2 - 25684074
AN - SCOPUS:84926407061
VL - 136
SP - 53
EP - 63
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
ER -