Hematopoietic activity in putative mouse primordial germ cell populations

Maria Lucia Scaldaferri, Francesca Gioia Klinger, Donatella Farini, Anna Di Carlo, Rita Carsetti, Ezio Giorda, Massimo De Felici

Research output: Contribution to journalArticle

Abstract

In the present paper, starting from the observation of heterogeneous expression of the GOF-18δPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFPweak CD-34weak/high cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.

Original languageEnglish
Pages (from-to)53-63
Number of pages11
JournalMechanisms of Development
Volume136
DOIs
Publication statusPublished - May 1 2015

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Keywords

  • Allantois
  • Germline
  • Hematopoiesis
  • Oct3/4
  • Primordial germ cells

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Medicine(all)

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