Hematopoietic progenitor cell collection and neoplastic cell contamination in breast cancer patients receiving chemotherapy plus granulocyte-colony stimulating factor (G-CSF) or G-CSF alone for mobilization

F. Bertolini, A. Lanza, F. Peccatori, C. Zibera, N. Gibelli, C. Perotti, G. A. Da Prada, L. Torretta, E. Cocorocchio, G. Martinelli, G. Robustelli Della Cuna

Research output: Contribution to journalArticlepeer-review

Abstract

Background: We compared hematopoietic progenitor cell (HPC) collection and neoplastic cell contamination in breast cancer patients given cyclophosphamide (CTX) plus granulocytecolony stimulating factor (G-CSF) or G-CSF alone for mobilization. Patients and methods: In 57 stage II-III breast cancer patients, CD34+ cells, colony-forming units-granulocyte macrophage (CFU-GM), early HPC and breast cancer cells were counted in HPC collections obtained after CTX plus G-CSF (n = 27) or G-CSF-alone mobilization (n = 30). Results: The CD34+ cell collection was about two-fold greater after CTX plus G-CSF mobilization (11.0 ± 7.9 vs. 5.8 ± 3.5 x 106/kg, P <0.001). Similarly, the total number of CFUGM, CD34+CD38- cells and of week-5 cobblestone area forming cells (CAFC) collected was significantly higher in patients mobilized with CTX plus G-CSF. Breast cancer cells were found in the apheresis products of 22% of patients mobilized with CTX plus G-CSF and in 10% of patients mobilized with G-CSF alone (P = 0.36). Of seven patients who failed G-CSF-alone mobilization and eventually underwent chemotherapy plus G- CSF mobilization, none had cytokeratin-positive cells after G-CSF mobilization, whereas four out of seven had cytokeratin-positive cells after chemotherapy plus G-CSF (P = 0.07 by χ2 test). Conclusion. The CTX plus G- CSF mobilization protocol was associated with a significantly higher HPC collection. However, this benefit was not accompanied by a reduction in the incidence of tumor-contaminated HPC graft.

Original languageEnglish
Pages (from-to)913-916
Number of pages4
JournalAnnals of Oncology
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 1998

Keywords

  • Breast cancer
  • G-CSF
  • Mobilization
  • Stem cells
  • Transplantation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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