Heme and sensory neuropathy

insights from novel mutations in the heme exporter FLVCR1

Francesca Bertino, Kyra Firestone, Emanuele Bellacchio, Kelly E Jackson, Alexander Asamoah, Joseph Hersh, Veronica Fiorito, Francesca Destefanis, Rusty Gonser, Megan E Tucker, Fiorella Altruda, Emanuela Tolosano, Deborah Chiabrando

Research output: Contribution to journalArticle

Abstract

Hereditary sensory and autonomic neuropathies (HSANs) are a group of clinically and genetically heterogeneous disorders of the peripheral nervous system mainly characterized by impaired nociception and autonomic dysfunction. We previously identified heme metabolism as a novel pathway contributing to sensory neurons maintenance and nociception. Indeed, we reported mutations in the Feline Leukemia Virus subgroup C Receptor 1 (FLVCR1) gene in individuals affected by HSAN. FLVCR1 gene encodes for two heme export proteins, FLVCR1a (plasma membrane) and FLVCR1b (mitochondria), crucially involved in the regulation of cellular heme homeostasis.Here, we report on two additional patients carrying novel biallelic mutations in FLVCR1 translation initiation codon (TIC) (c.2T>C; p. (Met1Thr), c.3G>T; p. (Met1Ile)). We overexpressed the c.2T>C; p. (Met1Thr) mutant in human cell lines and we describe its impact on protein structure and function in comparison with other HSAN related mutations. We found that the mutation interfere with translation in two different ways: by lowering levels of translation of wild-type protein and by inducing translation initiation from a downstream in frame ATG, leading to the production of a N-terminal truncated protein that is retained in the endoplasmic reticulum. The impact of different kinds of mutations on FLVCR1a localization and structure was also described.The identification of novel FLVCR1 mutations in HSAN reinforces the crucial role of heme in sensory neurons maintenance and pain perception. Moreover, our in vitro findings demonstrate that heme export is not completely lost in HSAN patients, thus suggesting the possibility to improve FLVCR1 expression/activity for therapeutic purposes.

Original languageEnglish
JournalPain
DOIs
Publication statusE-pub ahead of print - Aug 10 2019

Fingerprint

Feline Leukemia Virus
Hereditary Sensory and Autonomic Neuropathies
Heme
Mutation
Nociception
Sensory Receptor Cells
Translational Peptide Chain Initiation
Maintenance
Pain Perception
Proteins
Initiator Codon
Peripheral Nervous System Diseases
Endoplasmic Reticulum
Genes
Mitochondria
Homeostasis
Cell Membrane
Cell Line

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Heme and sensory neuropathy : insights from novel mutations in the heme exporter FLVCR1. / Bertino, Francesca; Firestone, Kyra; Bellacchio, Emanuele; Jackson, Kelly E; Asamoah, Alexander; Hersh, Joseph; Fiorito, Veronica; Destefanis, Francesca; Gonser, Rusty; Tucker, Megan E; Altruda, Fiorella; Tolosano, Emanuela; Chiabrando, Deborah.

In: Pain, 10.08.2019.

Research output: Contribution to journalArticle

Bertino, F, Firestone, K, Bellacchio, E, Jackson, KE, Asamoah, A, Hersh, J, Fiorito, V, Destefanis, F, Gonser, R, Tucker, ME, Altruda, F, Tolosano, E & Chiabrando, D 2019, 'Heme and sensory neuropathy: insights from novel mutations in the heme exporter FLVCR1', Pain. https://doi.org/10.1097/j.pain.0000000000001675
Bertino, Francesca ; Firestone, Kyra ; Bellacchio, Emanuele ; Jackson, Kelly E ; Asamoah, Alexander ; Hersh, Joseph ; Fiorito, Veronica ; Destefanis, Francesca ; Gonser, Rusty ; Tucker, Megan E ; Altruda, Fiorella ; Tolosano, Emanuela ; Chiabrando, Deborah. / Heme and sensory neuropathy : insights from novel mutations in the heme exporter FLVCR1. In: Pain. 2019.
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abstract = "Hereditary sensory and autonomic neuropathies (HSANs) are a group of clinically and genetically heterogeneous disorders of the peripheral nervous system mainly characterized by impaired nociception and autonomic dysfunction. We previously identified heme metabolism as a novel pathway contributing to sensory neurons maintenance and nociception. Indeed, we reported mutations in the Feline Leukemia Virus subgroup C Receptor 1 (FLVCR1) gene in individuals affected by HSAN. FLVCR1 gene encodes for two heme export proteins, FLVCR1a (plasma membrane) and FLVCR1b (mitochondria), crucially involved in the regulation of cellular heme homeostasis.Here, we report on two additional patients carrying novel biallelic mutations in FLVCR1 translation initiation codon (TIC) (c.2T>C; p. (Met1Thr), c.3G>T; p. (Met1Ile)). We overexpressed the c.2T>C; p. (Met1Thr) mutant in human cell lines and we describe its impact on protein structure and function in comparison with other HSAN related mutations. We found that the mutation interfere with translation in two different ways: by lowering levels of translation of wild-type protein and by inducing translation initiation from a downstream in frame ATG, leading to the production of a N-terminal truncated protein that is retained in the endoplasmic reticulum. The impact of different kinds of mutations on FLVCR1a localization and structure was also described.The identification of novel FLVCR1 mutations in HSAN reinforces the crucial role of heme in sensory neurons maintenance and pain perception. Moreover, our in vitro findings demonstrate that heme export is not completely lost in HSAN patients, thus suggesting the possibility to improve FLVCR1 expression/activity for therapeutic purposes.",
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AU - Bertino, Francesca

AU - Firestone, Kyra

AU - Bellacchio, Emanuele

AU - Jackson, Kelly E

AU - Asamoah, Alexander

AU - Hersh, Joseph

AU - Fiorito, Veronica

AU - Destefanis, Francesca

AU - Gonser, Rusty

AU - Tucker, Megan E

AU - Altruda, Fiorella

AU - Tolosano, Emanuela

AU - Chiabrando, Deborah

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N2 - Hereditary sensory and autonomic neuropathies (HSANs) are a group of clinically and genetically heterogeneous disorders of the peripheral nervous system mainly characterized by impaired nociception and autonomic dysfunction. We previously identified heme metabolism as a novel pathway contributing to sensory neurons maintenance and nociception. Indeed, we reported mutations in the Feline Leukemia Virus subgroup C Receptor 1 (FLVCR1) gene in individuals affected by HSAN. FLVCR1 gene encodes for two heme export proteins, FLVCR1a (plasma membrane) and FLVCR1b (mitochondria), crucially involved in the regulation of cellular heme homeostasis.Here, we report on two additional patients carrying novel biallelic mutations in FLVCR1 translation initiation codon (TIC) (c.2T>C; p. (Met1Thr), c.3G>T; p. (Met1Ile)). We overexpressed the c.2T>C; p. (Met1Thr) mutant in human cell lines and we describe its impact on protein structure and function in comparison with other HSAN related mutations. We found that the mutation interfere with translation in two different ways: by lowering levels of translation of wild-type protein and by inducing translation initiation from a downstream in frame ATG, leading to the production of a N-terminal truncated protein that is retained in the endoplasmic reticulum. The impact of different kinds of mutations on FLVCR1a localization and structure was also described.The identification of novel FLVCR1 mutations in HSAN reinforces the crucial role of heme in sensory neurons maintenance and pain perception. Moreover, our in vitro findings demonstrate that heme export is not completely lost in HSAN patients, thus suggesting the possibility to improve FLVCR1 expression/activity for therapeutic purposes.

AB - Hereditary sensory and autonomic neuropathies (HSANs) are a group of clinically and genetically heterogeneous disorders of the peripheral nervous system mainly characterized by impaired nociception and autonomic dysfunction. We previously identified heme metabolism as a novel pathway contributing to sensory neurons maintenance and nociception. Indeed, we reported mutations in the Feline Leukemia Virus subgroup C Receptor 1 (FLVCR1) gene in individuals affected by HSAN. FLVCR1 gene encodes for two heme export proteins, FLVCR1a (plasma membrane) and FLVCR1b (mitochondria), crucially involved in the regulation of cellular heme homeostasis.Here, we report on two additional patients carrying novel biallelic mutations in FLVCR1 translation initiation codon (TIC) (c.2T>C; p. (Met1Thr), c.3G>T; p. (Met1Ile)). We overexpressed the c.2T>C; p. (Met1Thr) mutant in human cell lines and we describe its impact on protein structure and function in comparison with other HSAN related mutations. We found that the mutation interfere with translation in two different ways: by lowering levels of translation of wild-type protein and by inducing translation initiation from a downstream in frame ATG, leading to the production of a N-terminal truncated protein that is retained in the endoplasmic reticulum. The impact of different kinds of mutations on FLVCR1a localization and structure was also described.The identification of novel FLVCR1 mutations in HSAN reinforces the crucial role of heme in sensory neurons maintenance and pain perception. Moreover, our in vitro findings demonstrate that heme export is not completely lost in HSAN patients, thus suggesting the possibility to improve FLVCR1 expression/activity for therapeutic purposes.

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