Abstract
Atrial fibrillation becomes a self-perpetuating arrhythmia as a consequence of electrophysiogic and structural remodeling involving the atrium. Oxidative stress may be a link between this rhythm disturbance and electrophysiogic remodeling. The aim of this study was to evaluate whether the heme oxygenase-1 (HO-1) marker of oxidative stress was more expressed in left atrial sites with stronger structural remodeling in patients affected by chronic atrial fibrillation (CAF) and mitral valve disease (MD). Myocardial samples were taken from the left atrial posterior wall (LAPW) and left atrial appendage (LAA) of 24 patients with CAF-MD in addition to 10 autopsy controls. The levels of HO-1 messenger RNA (mRNA) and HO-1 protein in each pathologic LAPW and LAA were quantified using reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Furthermore, light microscopy was used to morphometrically evaluate the differential myocyte and interstitial changes in the same CAF-MD LAPW and LAA samples. In controls, HO-1 protein was quantified using enzyme-linked immunosorbent assay. Unlike controls, patients with CAF-MD had higher levels of HO-1 mRNA and its protein product, expressed as LAPW/LAA ratios, in the LAPW (2.18 ± 1.18, P <.0001, and 1.55 ± 0.67, P <.005), and their LAPW also showed greater histologic changes in myocytolytic myocytes (15.1% ± 3.1% versus 6.9% ± 3.3%, P <.0001), interstitial fibrosis (8.2% ± 2.2% versus 2.8% ± 1.2%, P <.0001), and capillary density (816 ± 120 number/mm2 versus 1114 ± 188 number/mm2; P <.05). In addition, markers of oxidative stress were immunohistochemically studied with antinitrotyrosine and anti-iNOS antibodies. In patients with CAF-MD, the inducible enzyme HO-1 is more expressed in the left atrial areas that show greater structural remodeling. This finding strongly suggests a pathogenetic relationship between oxidative stress and the degree of histologic change.
| Original language | English |
|---|---|
| Pages (from-to) | 1162-1171 |
| Number of pages | 10 |
| Journal | Human Pathology |
| Volume | 39 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2008 |
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Keywords
- Atrial fibrillation
- Heme oxygenase-1
- Mitral valve disease
- Oxidative stress
- Structural remodeling
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Heme oxygenase-1 expression in the left atrial myocardium of patients with chronic atrial fibrillation related to mitral valve disease : its regional relationship with structural remodeling. / Corradi, Domenico; Callegari, Sergio; Maestri, Roberta; Benussi, Stefano; Bosio, Silvia; De Palma, Giuseppe; Alinovi, Rossella; Caglieri, Andrea; Goldoni, Matteo; Mozzoni, Paola; Pastori, Paolo; Manotti, Laura; Nascimbene, Simona; Dorigo, Enrica; Rusconi, Raffaella; Astorri, Ettore; Alfieri, Ottavio.
In: Human Pathology, Vol. 39, No. 8, 08.2008, p. 1162-1171.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heme oxygenase-1 expression in the left atrial myocardium of patients with chronic atrial fibrillation related to mitral valve disease
T2 - its regional relationship with structural remodeling
AU - Corradi, Domenico
AU - Callegari, Sergio
AU - Maestri, Roberta
AU - Benussi, Stefano
AU - Bosio, Silvia
AU - De Palma, Giuseppe
AU - Alinovi, Rossella
AU - Caglieri, Andrea
AU - Goldoni, Matteo
AU - Mozzoni, Paola
AU - Pastori, Paolo
AU - Manotti, Laura
AU - Nascimbene, Simona
AU - Dorigo, Enrica
AU - Rusconi, Raffaella
AU - Astorri, Ettore
AU - Alfieri, Ottavio
PY - 2008/8
Y1 - 2008/8
N2 - Atrial fibrillation becomes a self-perpetuating arrhythmia as a consequence of electrophysiogic and structural remodeling involving the atrium. Oxidative stress may be a link between this rhythm disturbance and electrophysiogic remodeling. The aim of this study was to evaluate whether the heme oxygenase-1 (HO-1) marker of oxidative stress was more expressed in left atrial sites with stronger structural remodeling in patients affected by chronic atrial fibrillation (CAF) and mitral valve disease (MD). Myocardial samples were taken from the left atrial posterior wall (LAPW) and left atrial appendage (LAA) of 24 patients with CAF-MD in addition to 10 autopsy controls. The levels of HO-1 messenger RNA (mRNA) and HO-1 protein in each pathologic LAPW and LAA were quantified using reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Furthermore, light microscopy was used to morphometrically evaluate the differential myocyte and interstitial changes in the same CAF-MD LAPW and LAA samples. In controls, HO-1 protein was quantified using enzyme-linked immunosorbent assay. Unlike controls, patients with CAF-MD had higher levels of HO-1 mRNA and its protein product, expressed as LAPW/LAA ratios, in the LAPW (2.18 ± 1.18, P <.0001, and 1.55 ± 0.67, P <.005), and their LAPW also showed greater histologic changes in myocytolytic myocytes (15.1% ± 3.1% versus 6.9% ± 3.3%, P <.0001), interstitial fibrosis (8.2% ± 2.2% versus 2.8% ± 1.2%, P <.0001), and capillary density (816 ± 120 number/mm2 versus 1114 ± 188 number/mm2; P <.05). In addition, markers of oxidative stress were immunohistochemically studied with antinitrotyrosine and anti-iNOS antibodies. In patients with CAF-MD, the inducible enzyme HO-1 is more expressed in the left atrial areas that show greater structural remodeling. This finding strongly suggests a pathogenetic relationship between oxidative stress and the degree of histologic change.
AB - Atrial fibrillation becomes a self-perpetuating arrhythmia as a consequence of electrophysiogic and structural remodeling involving the atrium. Oxidative stress may be a link between this rhythm disturbance and electrophysiogic remodeling. The aim of this study was to evaluate whether the heme oxygenase-1 (HO-1) marker of oxidative stress was more expressed in left atrial sites with stronger structural remodeling in patients affected by chronic atrial fibrillation (CAF) and mitral valve disease (MD). Myocardial samples were taken from the left atrial posterior wall (LAPW) and left atrial appendage (LAA) of 24 patients with CAF-MD in addition to 10 autopsy controls. The levels of HO-1 messenger RNA (mRNA) and HO-1 protein in each pathologic LAPW and LAA were quantified using reverse transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Furthermore, light microscopy was used to morphometrically evaluate the differential myocyte and interstitial changes in the same CAF-MD LAPW and LAA samples. In controls, HO-1 protein was quantified using enzyme-linked immunosorbent assay. Unlike controls, patients with CAF-MD had higher levels of HO-1 mRNA and its protein product, expressed as LAPW/LAA ratios, in the LAPW (2.18 ± 1.18, P <.0001, and 1.55 ± 0.67, P <.005), and their LAPW also showed greater histologic changes in myocytolytic myocytes (15.1% ± 3.1% versus 6.9% ± 3.3%, P <.0001), interstitial fibrosis (8.2% ± 2.2% versus 2.8% ± 1.2%, P <.0001), and capillary density (816 ± 120 number/mm2 versus 1114 ± 188 number/mm2; P <.05). In addition, markers of oxidative stress were immunohistochemically studied with antinitrotyrosine and anti-iNOS antibodies. In patients with CAF-MD, the inducible enzyme HO-1 is more expressed in the left atrial areas that show greater structural remodeling. This finding strongly suggests a pathogenetic relationship between oxidative stress and the degree of histologic change.
KW - Atrial fibrillation
KW - Heme oxygenase-1
KW - Mitral valve disease
KW - Oxidative stress
KW - Structural remodeling
UR - http://www.scopus.com/inward/record.url?scp=47849121158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47849121158&partnerID=8YFLogxK
U2 - 10.1016/j.humpath.2007.12.007
DO - 10.1016/j.humpath.2007.12.007
M3 - Article
C2 - 18440590
AN - SCOPUS:47849121158
VL - 39
SP - 1162
EP - 1171
JO - Human Pathology
JF - Human Pathology
SN - 0046-8177
IS - 8
ER -