Abstract
Lung cancer causes more deaths worldwide than any other cancer. In addition to cigarette smoking, dietary factors may contribute to lung carcinogenesis. Epidemiologic studies, including the environment and genetics in lung cancer etiology (EAGLE), have reported increased consumption of red/processed meats to be associated with higher risk of lung cancer. Heme-iron toxicity may link meat intake with cancer. We investigated this hypothesis in meat-related lung carcinogenesis using whole genome expression. We measured genome-wide expression (HG-U133A) in 49 tumor and 42 non-involved fresh frozen lung tissues of 64 adenocarcinoma EAGLE patients. We studied gene expression profiles by high-versus-low meat consumption, with and without adjustment by sex, age, and smoking. Threshold for significance was a false discovery rate (FDR) ≤0.15. We studied whether the identified genes played a role in heme-iron related processes by means of manually curated literature search and gene ontology-based pathway analysis. We found that gene expression of 232 annotated genes in tumor tissue significantly distinguished lung adenocarcinoma cases who consumed above/below the median intake of fresh red meats (FDR=0.12). Sixty-three (∼28%) of the 232 identified genes (12 expected by chance, P-value
| Original language | English |
|---|---|
| Pages (from-to) | 548-556 |
| Number of pages | 9 |
| Journal | Molecular Carcinogenesis |
| Volume | 53 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2014 |
Fingerprint
Keywords
- Gene expression
- Heme-iron
- Lung cancer
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
Cite this
Heme-related gene expression signatures of meat intakes in lung cancer tissues. / Lam, Tram Kim; Rotunno, Melissa; Ryan, Brid M.; Pesatori, Angela C.; Bertazzi, Pier Alberto; Spitz, Margaret; Caporaso, Neil E.; Landi, Maria Teresa.
In: Molecular Carcinogenesis, Vol. 53, No. 7, 2014, p. 548-556.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heme-related gene expression signatures of meat intakes in lung cancer tissues
AU - Lam, Tram Kim
AU - Rotunno, Melissa
AU - Ryan, Brid M.
AU - Pesatori, Angela C.
AU - Bertazzi, Pier Alberto
AU - Spitz, Margaret
AU - Caporaso, Neil E.
AU - Landi, Maria Teresa
PY - 2014
Y1 - 2014
N2 - Lung cancer causes more deaths worldwide than any other cancer. In addition to cigarette smoking, dietary factors may contribute to lung carcinogenesis. Epidemiologic studies, including the environment and genetics in lung cancer etiology (EAGLE), have reported increased consumption of red/processed meats to be associated with higher risk of lung cancer. Heme-iron toxicity may link meat intake with cancer. We investigated this hypothesis in meat-related lung carcinogenesis using whole genome expression. We measured genome-wide expression (HG-U133A) in 49 tumor and 42 non-involved fresh frozen lung tissues of 64 adenocarcinoma EAGLE patients. We studied gene expression profiles by high-versus-low meat consumption, with and without adjustment by sex, age, and smoking. Threshold for significance was a false discovery rate (FDR) ≤0.15. We studied whether the identified genes played a role in heme-iron related processes by means of manually curated literature search and gene ontology-based pathway analysis. We found that gene expression of 232 annotated genes in tumor tissue significantly distinguished lung adenocarcinoma cases who consumed above/below the median intake of fresh red meats (FDR=0.12). Sixty-three (∼28%) of the 232 identified genes (12 expected by chance, P-value
AB - Lung cancer causes more deaths worldwide than any other cancer. In addition to cigarette smoking, dietary factors may contribute to lung carcinogenesis. Epidemiologic studies, including the environment and genetics in lung cancer etiology (EAGLE), have reported increased consumption of red/processed meats to be associated with higher risk of lung cancer. Heme-iron toxicity may link meat intake with cancer. We investigated this hypothesis in meat-related lung carcinogenesis using whole genome expression. We measured genome-wide expression (HG-U133A) in 49 tumor and 42 non-involved fresh frozen lung tissues of 64 adenocarcinoma EAGLE patients. We studied gene expression profiles by high-versus-low meat consumption, with and without adjustment by sex, age, and smoking. Threshold for significance was a false discovery rate (FDR) ≤0.15. We studied whether the identified genes played a role in heme-iron related processes by means of manually curated literature search and gene ontology-based pathway analysis. We found that gene expression of 232 annotated genes in tumor tissue significantly distinguished lung adenocarcinoma cases who consumed above/below the median intake of fresh red meats (FDR=0.12). Sixty-three (∼28%) of the 232 identified genes (12 expected by chance, P-value
KW - Gene expression
KW - Heme-iron
KW - Lung cancer
UR - http://www.scopus.com/inward/record.url?scp=84902550113&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902550113&partnerID=8YFLogxK
U2 - 10.1002/mc.22006
DO - 10.1002/mc.22006
M3 - Article
C2 - 23681825
AN - SCOPUS:84902550113
VL - 53
SP - 548
EP - 556
JO - Molecular Carcinogenesis
JF - Molecular Carcinogenesis
SN - 0899-1987
IS - 7
ER -