Hemocyanin subunit organization of the gastropod Rapana thomasiana

Wolfgang Gebauer, Stanka Stoeva, Wolfgang Voelter, Enrico Dainese, Benedetto Salvato, Mariano Beltramini, Jürgen Markl

Research output: Contribution to journalArticle

Abstract

RtH1 and RtH2, the two hemocyanin isoforms of the prosobranch gastropod Rapana thomasiana, have been purified by anion-exchange chromatography and studied by SDS-PAGE and immunoelectrophoresis. Both subunit types are built up of eight functional units (FUs). Under reducing conditions subunit RtH2 splits into two fragments, RtH2-α-f and RtH2-gh, suggesting the presence of a disulfide bridge between FU2-f and FU2-g. By proteolytic cleavage of the subunits into three-, two-, and single-FU fragments, purification of fragments by HPLC, N-terminal sequencing of the peptides, and crossed-line immunoelectrophoresis, FUs-α-h of RtH2 and FU-α, FU-d, FU-e, and FU-f of RtH1 were identified and correlated to the eight-FUs pattern of immunoelectrophoresis. FU-α, FU-e, and FU-f of RtH1 and RtH2 are very closely related immunologically. RtH1 and RtH2 both correspond immunologically to KLH2, one of the two hemocyanin isoforms of the prosobranch gastropod Megathura crenulata.

Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume372
Issue number1
DOIs
Publication statusPublished - Dec 1 1999

Fingerprint

Hemocyanin
Immunoelectrophoresis
Gastropoda
Protein Isoforms
Two-Dimensional Immunoelectrophoresis
Disulfides
Anions
Chromatography
Polyacrylamide Gel Electrophoresis
High Pressure Liquid Chromatography
Peptides
Purification

Keywords

  • Disulfide bridge
  • Hemocyanin
  • Mollusca
  • Rapana thomasiana
  • Subunit structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Gebauer, W., Stoeva, S., Voelter, W., Dainese, E., Salvato, B., Beltramini, M., & Markl, J. (1999). Hemocyanin subunit organization of the gastropod Rapana thomasiana. Archives of Biochemistry and Biophysics, 372(1), 128-134. https://doi.org/10.1006/abbi.1999.1478

Hemocyanin subunit organization of the gastropod Rapana thomasiana. / Gebauer, Wolfgang; Stoeva, Stanka; Voelter, Wolfgang; Dainese, Enrico; Salvato, Benedetto; Beltramini, Mariano; Markl, Jürgen.

In: Archives of Biochemistry and Biophysics, Vol. 372, No. 1, 01.12.1999, p. 128-134.

Research output: Contribution to journalArticle

Gebauer, W, Stoeva, S, Voelter, W, Dainese, E, Salvato, B, Beltramini, M & Markl, J 1999, 'Hemocyanin subunit organization of the gastropod Rapana thomasiana', Archives of Biochemistry and Biophysics, vol. 372, no. 1, pp. 128-134. https://doi.org/10.1006/abbi.1999.1478
Gebauer W, Stoeva S, Voelter W, Dainese E, Salvato B, Beltramini M et al. Hemocyanin subunit organization of the gastropod Rapana thomasiana. Archives of Biochemistry and Biophysics. 1999 Dec 1;372(1):128-134. https://doi.org/10.1006/abbi.1999.1478
Gebauer, Wolfgang ; Stoeva, Stanka ; Voelter, Wolfgang ; Dainese, Enrico ; Salvato, Benedetto ; Beltramini, Mariano ; Markl, Jürgen. / Hemocyanin subunit organization of the gastropod Rapana thomasiana. In: Archives of Biochemistry and Biophysics. 1999 ; Vol. 372, No. 1. pp. 128-134.
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