TY - JOUR
T1 - Hemogenic endothelium generates mesoangioblasts that contribute to several mesodermal lineages in vivo
AU - Azzoni, Emanuele
AU - Conti, Valentina
AU - Campana, Lara
AU - Dellavalle, Arianna
AU - Adams, Ralf H.
AU - Cossu, Giulio
AU - Brunelli, Silvia
PY - 2014
Y1 - 2014
N2 - The embryonic endothelium is a known source of hematopoietic stem cells. Moreover, vessel-associated progenitors/stem cells with multilineage mesodermal differentiation potential, such as the 'embryonic mesoangioblasts', originate in vitro from the endothelium. Using a genetic lineage tracing approach, we show that early extra-embryonic endothelium generates, in a narrow time-window and prior to the hemogenic endothelium in the major embryonic arteries, hematopoietic cells that migrate to the embryo proper, and are subsequently found within the mesenchyme. A subpopulation of these cells, distinct from embryonic macrophages, co-expresses mesenchymal and hematopoietic markers. In addition, hemogenic endothelium-derived cells contribute to skeletal and smooth muscle, and to other mesodermal cells in vivo, and display features of embryonic mesoangioblasts in vitro. Therefore, we provide new insights on the distinctive characteristics of the extra-embryonic and embryonic hemogenic endothelium, and we identify the putative in vivo counterpart of embryonic mesoangioblasts, suggesting their identity and developmental ontogeny.
AB - The embryonic endothelium is a known source of hematopoietic stem cells. Moreover, vessel-associated progenitors/stem cells with multilineage mesodermal differentiation potential, such as the 'embryonic mesoangioblasts', originate in vitro from the endothelium. Using a genetic lineage tracing approach, we show that early extra-embryonic endothelium generates, in a narrow time-window and prior to the hemogenic endothelium in the major embryonic arteries, hematopoietic cells that migrate to the embryo proper, and are subsequently found within the mesenchyme. A subpopulation of these cells, distinct from embryonic macrophages, co-expresses mesenchymal and hematopoietic markers. In addition, hemogenic endothelium-derived cells contribute to skeletal and smooth muscle, and to other mesodermal cells in vivo, and display features of embryonic mesoangioblasts in vitro. Therefore, we provide new insights on the distinctive characteristics of the extra-embryonic and embryonic hemogenic endothelium, and we identify the putative in vivo counterpart of embryonic mesoangioblasts, suggesting their identity and developmental ontogeny.
KW - Hemogenic endothelium
KW - Mesoangioblasts
KW - Mouse
KW - Muscle
KW - VE-Cadherin
UR - http://www.scopus.com/inward/record.url?scp=84899636141&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899636141&partnerID=8YFLogxK
U2 - 10.1242/dev.103242
DO - 10.1242/dev.103242
M3 - Article
C2 - 24757004
AN - SCOPUS:84899636141
VL - 141
SP - 1821
EP - 1834
JO - Development (Cambridge)
JF - Development (Cambridge)
SN - 0950-1991
IS - 9
ER -