Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners

Petra Begemann, Patricia B. Upton, Asoka Ranasinghe, James A. Swenberg, Leonardo Soleo, Luigi Vimercati, Alfonso Gelormini, Silvia Fustinoni, Iris Zwirner-Baier, Hans Günter Neumann

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hemoglobin adducts were determined as biomarkers of 1,3-butadiene (BD) in 30 workers and 10 controls from an Italian BD plant and in 14 diesel-exposed miners. N-(2,3,4-trihydroxybutyl)valine (THBVal), an N-terminal valine globin adduct of reactive butadiene metabolites, was analyzed by gas chromatography/high resolution mass spectrometry after a modified Edman degradation and further acetylation. The BD exposure for the plant workers was 31 μg/m3 (personal sampling). Whereas there was no detectable difference in hemoglobin adduct levels (range 17.7-61.4 pmol/g globin) between the total group of exposed and controls, slight but significant differences could be found between two subgroups of workers from different production units as well as one subgroup and controls (P <0.05), between smoking (n = 13) and non-smoking exposed workers (n = 17; P = 0.066) as well as between smoking exposed workers and controls (P = 0.055). Adduct levels of the miners (all non-smokers) were in the same range as those of the Italian BD-workers and controls. The internal exposure and strain measured by THBVal levels resulting from a very low occupational BD exposure was in the range of the contribution of moderate smoking.

Original languageEnglish
Pages (from-to)675-678
Number of pages4
JournalChemico-Biological Interactions
Volume135-136
DOIs
Publication statusPublished - Jun 1 2001

Fingerprint

Miners
Biomarkers
Hemoglobins
Globins
Smoking
Acetylation
Valine
Occupational Exposure
Metabolites
Gas chromatography
Gas Chromatography
Mass spectrometry
1,3-butadiene
Mass Spectrometry
Sampling
Degradation
Control Groups

Keywords

  • Biomonitoring
  • Butadiene
  • Epoxybutanediol
  • Hemoglobin adducts

ASJC Scopus subject areas

  • Toxicology

Cite this

Begemann, P., Upton, P. B., Ranasinghe, A., Swenberg, J. A., Soleo, L., Vimercati, L., ... Neumann, H. G. (2001). Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners. Chemico-Biological Interactions, 135-136, 675-678. https://doi.org/10.1016/S0009-2797(01)00207-1

Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners. / Begemann, Petra; Upton, Patricia B.; Ranasinghe, Asoka; Swenberg, James A.; Soleo, Leonardo; Vimercati, Luigi; Gelormini, Alfonso; Fustinoni, Silvia; Zwirner-Baier, Iris; Neumann, Hans Günter.

In: Chemico-Biological Interactions, Vol. 135-136, 01.06.2001, p. 675-678.

Research output: Contribution to journalArticle

Begemann, P, Upton, PB, Ranasinghe, A, Swenberg, JA, Soleo, L, Vimercati, L, Gelormini, A, Fustinoni, S, Zwirner-Baier, I & Neumann, HG 2001, 'Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners', Chemico-Biological Interactions, vol. 135-136, pp. 675-678. https://doi.org/10.1016/S0009-2797(01)00207-1
Begemann, Petra ; Upton, Patricia B. ; Ranasinghe, Asoka ; Swenberg, James A. ; Soleo, Leonardo ; Vimercati, Luigi ; Gelormini, Alfonso ; Fustinoni, Silvia ; Zwirner-Baier, Iris ; Neumann, Hans Günter. / Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners. In: Chemico-Biological Interactions. 2001 ; Vol. 135-136. pp. 675-678.
@article{d41a2eaa4c1f40d3954a8820f9097cdc,
title = "Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners",
abstract = "Hemoglobin adducts were determined as biomarkers of 1,3-butadiene (BD) in 30 workers and 10 controls from an Italian BD plant and in 14 diesel-exposed miners. N-(2,3,4-trihydroxybutyl)valine (THBVal), an N-terminal valine globin adduct of reactive butadiene metabolites, was analyzed by gas chromatography/high resolution mass spectrometry after a modified Edman degradation and further acetylation. The BD exposure for the plant workers was 31 μg/m3 (personal sampling). Whereas there was no detectable difference in hemoglobin adduct levels (range 17.7-61.4 pmol/g globin) between the total group of exposed and controls, slight but significant differences could be found between two subgroups of workers from different production units as well as one subgroup and controls (P <0.05), between smoking (n = 13) and non-smoking exposed workers (n = 17; P = 0.066) as well as between smoking exposed workers and controls (P = 0.055). Adduct levels of the miners (all non-smokers) were in the same range as those of the Italian BD-workers and controls. The internal exposure and strain measured by THBVal levels resulting from a very low occupational BD exposure was in the range of the contribution of moderate smoking.",
keywords = "Biomonitoring, Butadiene, Epoxybutanediol, Hemoglobin adducts",
author = "Petra Begemann and Upton, {Patricia B.} and Asoka Ranasinghe and Swenberg, {James A.} and Leonardo Soleo and Luigi Vimercati and Alfonso Gelormini and Silvia Fustinoni and Iris Zwirner-Baier and Neumann, {Hans G{\"u}nter}",
year = "2001",
month = "6",
day = "1",
doi = "10.1016/S0009-2797(01)00207-1",
language = "English",
volume = "135-136",
pages = "675--678",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners

AU - Begemann, Petra

AU - Upton, Patricia B.

AU - Ranasinghe, Asoka

AU - Swenberg, James A.

AU - Soleo, Leonardo

AU - Vimercati, Luigi

AU - Gelormini, Alfonso

AU - Fustinoni, Silvia

AU - Zwirner-Baier, Iris

AU - Neumann, Hans Günter

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Hemoglobin adducts were determined as biomarkers of 1,3-butadiene (BD) in 30 workers and 10 controls from an Italian BD plant and in 14 diesel-exposed miners. N-(2,3,4-trihydroxybutyl)valine (THBVal), an N-terminal valine globin adduct of reactive butadiene metabolites, was analyzed by gas chromatography/high resolution mass spectrometry after a modified Edman degradation and further acetylation. The BD exposure for the plant workers was 31 μg/m3 (personal sampling). Whereas there was no detectable difference in hemoglobin adduct levels (range 17.7-61.4 pmol/g globin) between the total group of exposed and controls, slight but significant differences could be found between two subgroups of workers from different production units as well as one subgroup and controls (P <0.05), between smoking (n = 13) and non-smoking exposed workers (n = 17; P = 0.066) as well as between smoking exposed workers and controls (P = 0.055). Adduct levels of the miners (all non-smokers) were in the same range as those of the Italian BD-workers and controls. The internal exposure and strain measured by THBVal levels resulting from a very low occupational BD exposure was in the range of the contribution of moderate smoking.

AB - Hemoglobin adducts were determined as biomarkers of 1,3-butadiene (BD) in 30 workers and 10 controls from an Italian BD plant and in 14 diesel-exposed miners. N-(2,3,4-trihydroxybutyl)valine (THBVal), an N-terminal valine globin adduct of reactive butadiene metabolites, was analyzed by gas chromatography/high resolution mass spectrometry after a modified Edman degradation and further acetylation. The BD exposure for the plant workers was 31 μg/m3 (personal sampling). Whereas there was no detectable difference in hemoglobin adduct levels (range 17.7-61.4 pmol/g globin) between the total group of exposed and controls, slight but significant differences could be found between two subgroups of workers from different production units as well as one subgroup and controls (P <0.05), between smoking (n = 13) and non-smoking exposed workers (n = 17; P = 0.066) as well as between smoking exposed workers and controls (P = 0.055). Adduct levels of the miners (all non-smokers) were in the same range as those of the Italian BD-workers and controls. The internal exposure and strain measured by THBVal levels resulting from a very low occupational BD exposure was in the range of the contribution of moderate smoking.

KW - Biomonitoring

KW - Butadiene

KW - Epoxybutanediol

KW - Hemoglobin adducts

UR - http://www.scopus.com/inward/record.url?scp=0035369783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035369783&partnerID=8YFLogxK

U2 - 10.1016/S0009-2797(01)00207-1

DO - 10.1016/S0009-2797(01)00207-1

M3 - Article

C2 - 11397421

AN - SCOPUS:0035369783

VL - 135-136

SP - 675

EP - 678

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

ER -