Hemoglobin adducts as biomarkers of 1,3-butadiene in occupationally low exposed Italian workers and a few diesel-exposed miners

Petra Begemann, Patricia B. Upton, Asoka Ranasinghe, James A. Swenberg, Leonardo Soleo, Luigi Vimercati, Alfonso Gelormini, Silvia Fustinoni, Iris Zwirner-Baier, Hans Günter Neumann

Research output: Contribution to journalArticlepeer-review

Abstract

Hemoglobin adducts were determined as biomarkers of 1,3-butadiene (BD) in 30 workers and 10 controls from an Italian BD plant and in 14 diesel-exposed miners. N-(2,3,4-trihydroxybutyl)valine (THBVal), an N-terminal valine globin adduct of reactive butadiene metabolites, was analyzed by gas chromatography/high resolution mass spectrometry after a modified Edman degradation and further acetylation. The BD exposure for the plant workers was 31 μg/m3 (personal sampling). Whereas there was no detectable difference in hemoglobin adduct levels (range 17.7-61.4 pmol/g globin) between the total group of exposed and controls, slight but significant differences could be found between two subgroups of workers from different production units as well as one subgroup and controls (P <0.05), between smoking (n = 13) and non-smoking exposed workers (n = 17; P = 0.066) as well as between smoking exposed workers and controls (P = 0.055). Adduct levels of the miners (all non-smokers) were in the same range as those of the Italian BD-workers and controls. The internal exposure and strain measured by THBVal levels resulting from a very low occupational BD exposure was in the range of the contribution of moderate smoking.

Original languageEnglish
Pages (from-to)675-678
Number of pages4
JournalChemico-Biological Interactions
Volume135-136
DOIs
Publication statusPublished - Jun 1 2001

Keywords

  • Biomonitoring
  • Butadiene
  • Epoxybutanediol
  • Hemoglobin adducts

ASJC Scopus subject areas

  • Toxicology

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