Hemopoiesis in healthy old people and centenarians: Well-maintained responsiveness of CD34+ cells to hemopoietic growth factors and remodeling of cytokine network

Gian Paolo Bagnara, Laura Bonsi, Pierluigi Strippoli, Francesca Bonifazi, Roberto Tonelli, Sergio D'Addato, Roberto Paganelli, Enrico Scala, Umberto Fagiolo, Daniela Monti, Andrea Cossarizza, Massimiliano Bonafé, Claudio Franceschi

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro hemopoiesis and hemopoietic cytokines production were evaluated in 9 centenarians (median age 100.5 years, age range: 100-104 years), 10 old people (median age: 71 years, age range: 66-73 years), and 10 young people (median age: 35 years, age range: 30-45 years), all carefully selected for their healthy status. The main findings were the following: (i) a trend towards a decreased absolute number of CD34+ progenitor cells in the peripheral blood of old people and centenarians, in comparison to young subjects; (ii) a well-preserved capability of CD34+ cells from old people and centenarians to respond to hemopoietic cytokines, and to form erythroid (BFU- E), granulocyte-macrophagic (CFU-GM), and mixed colonies (CFU-GEMM) in a way (number, size, and morphology) indistinguishable from that of young subjects; (iii) an age-related decreased in vitro production of granulocyte-macrophagic colony-stimulating factor (GM-CSF) and a decreased production of interleukin- 3 (IL-3) in centenarians by phytohemagglutinin (PHA)-stimulated peripheral blood mononuclear cells (PBMC); (iv) a linear increase of the serum level of stem cell factor (SCF), measured in the above-mentioned subjects and in 65 additional subjects, including 4 centenarians. These data suggest that basal hematopoietic potential is well preserved in healthy centenarians, and that the hemopoietic cytokine network undergoes a complex remodeling with age.

Original languageEnglish
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume55
Issue number2
Publication statusPublished - Feb 2000

ASJC Scopus subject areas

  • Ageing

Fingerprint

Dive into the research topics of 'Hemopoiesis in healthy old people and centenarians: Well-maintained responsiveness of CD34+ cells to hemopoietic growth factors and remodeling of cytokine network'. Together they form a unique fingerprint.

Cite this