Heparin protection against Fe2+-and Cu2+-mediated oxidation of liposomes

Riccardo Albertini, Simonetta Rindi, Alberto Passi, Giosue Pallavicini, Giancarlo De Luca

Research output: Contribution to journalArticle

Abstract

Heparin (HE) exhibited a protective effect on liposome peroxidation induced by Fe2+ and Cu2+, decreasing the formation of both conjugated dienes and thiobarbituric acid reactive substances (TBARS) in a dose-dependent manner. The antioxidant activity was more relevant in the oxidizing system employing Fe2+ and H2O2 and generating the highly reactive OH radical. The analysis of liposome size distribution by quasi-elastic laser light scattering showed that: (1) the native structure of the particles was completely lost after exposure to Fenton reagent; (2) the presence of HE in the reaction mixture completely prevented the peroxidative damage on liposomes. Thus, HE acts as an antioxidant factor on membrane lipid bilayer. This suggests that HE, released from mast-cell granules during inflammatory processes, might locally protect the cell membrane from the oxidative injuries.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalFEBS Letters
Volume383
Issue number3
DOIs
Publication statusPublished - Apr 1 1996

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Keywords

  • Copper oxidation
  • Heparin
  • Iron oxidation
  • Liposome

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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