Hepatic stellate cell immunodetection and cirrhotic evolution of viral hepatitis in liver allografts

Maria Guido, Massimo Rugge, Gioacchino Leandro, Isabel M. Fiel, Swan N. Thung

Research output: Contribution to journalArticle

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Abstract

Patients with chronic viral hepatitis are at high risk of developing cirrhosis, but the outcome of the disease in a given patient is unpredictable. Hepatic stellate cells have been demonstrated to be the most important cell type involved in hepatic fibrogenesis, regardless of the cause of the liver injury. The alpha isotype of actin (a phenotypic marker of smooth muscle cells) may be expressed by hepatic stellate cells, reflecting their 'activation' to myofibroblast-like cells. The present study aimed to analyze the expression of α-smooth muscle actin-positive hepatic stellate cells in liver allografts with recurrent viral hepatitis, and to evaluate whether and how such expression may be related to the outcome of the disease. Using immunohistochemistry and a semi-quantitative scoring system, the expression of α-smooth muscle actin in hepatic stellate cells was analyzed in liver allografts of 17 patients with recurrent vital hepatitis. They included nine patients who developed cirrhosis at the end of follow-up (mean time 23.6 months), and eight patients with no cirrhosis at the end of a comparable follow-up time (mean 30.1 months). In all patients, liver biopsy specimens were obtained between 3 and 6 months (t1) and between 10 and 15 months (t2) after transplantation. Preperfusion biopsy specimens of donor livers served as a baseline (t0). By comparison with the baseline biopsy, an increased number of α-smooth muscle actin-expressing hepatic stellate cells was observed in all cases in t1 biopsies. An increase in the amount of α- smooth muscle actin-positive hepatic stellate cells in zone i at h was significantly (P <.006) related to subsequent cirrhotic evolution. In conclusion, in liver allografts with recurrent viral hepatitis, the activation of hepatic stellate cells is an early event. An increased number of α-smooth muscle actin-positive hepatic stellate cells in zone 1 may represent an unfavorable event related to cirrhotic evolution.

Original languageEnglish
Pages (from-to)310-314
Number of pages5
JournalHepatology
Volume26
Issue number2
DOIs
Publication statusPublished - 1997

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Hepatic Stellate Cells
Hepatitis
Allografts
Actins
Liver
Smooth Muscle
Biopsy
Fibrosis
Virus Activation
Myofibroblasts
Chronic Hepatitis
Smooth Muscle Myocytes
Transplantation
Immunohistochemistry
Tissue Donors
Wounds and Injuries

ASJC Scopus subject areas

  • Hepatology

Cite this

Hepatic stellate cell immunodetection and cirrhotic evolution of viral hepatitis in liver allografts. / Guido, Maria; Rugge, Massimo; Leandro, Gioacchino; Fiel, Isabel M.; Thung, Swan N.

In: Hepatology, Vol. 26, No. 2, 1997, p. 310-314.

Research output: Contribution to journalArticle

Guido, Maria ; Rugge, Massimo ; Leandro, Gioacchino ; Fiel, Isabel M. ; Thung, Swan N. / Hepatic stellate cell immunodetection and cirrhotic evolution of viral hepatitis in liver allografts. In: Hepatology. 1997 ; Vol. 26, No. 2. pp. 310-314.
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