Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice

Luca G. Guidotti, Violeta Martinez, Ying Tsu Loh, Charles E. Rogler, Francis V. Chisari

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Transgenic mice that express the hepatitis B virus core protein were used to examine factors that influence the intracellular localization of nucleocapsid particles in the primary hepatocyte in vivo. In this model, viral nucleocapsid particles are strictly localized to the nucleus of the hepatocyte except when the nuclear membrane dissolves during cell division, at which time they enter the cytoplasm. The cytoplasmic nucleocapsid particles do not reenter the nucleus, however, when the nuclear membrane re- forms after cell division. The data support the notion that nucleocapsid particles can form de novo within the nucleus, and they suggest that preformed nucleocapsid particles cannot be transported across the intact nuclear membrane in either direction. The results imply that nucleocapsid disassembly is probably required for entry of the hepadnaviral genome into the nucleus, and they question the role of the intranuclear viral nucleocapsid particle during the viral life cycle.

Original languageEnglish
Pages (from-to)5469-5475
Number of pages7
JournalJournal of Virology
Volume68
Issue number9
Publication statusPublished - Sep 1994

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Nucleocapsid
nucleocapsid
Hepatitis B virus
Nuclear Envelope
nuclear membrane
Virion
Transgenic Mice
hepatocytes
Hepatocytes
genetically modified organisms
mice
Cell Division
cell division
Viral Core Proteins
Life Cycle Stages
virion
life cycle (organisms)
Cytoplasm
cytoplasm
Genome

ASJC Scopus subject areas

  • Immunology

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Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice. / Guidotti, Luca G.; Martinez, Violeta; Loh, Ying Tsu; Rogler, Charles E.; Chisari, Francis V.

In: Journal of Virology, Vol. 68, No. 9, 09.1994, p. 5469-5475.

Research output: Contribution to journalArticle

Guidotti, LG, Martinez, V, Loh, YT, Rogler, CE & Chisari, FV 1994, 'Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice', Journal of Virology, vol. 68, no. 9, pp. 5469-5475.
Guidotti, Luca G. ; Martinez, Violeta ; Loh, Ying Tsu ; Rogler, Charles E. ; Chisari, Francis V. / Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice. In: Journal of Virology. 1994 ; Vol. 68, No. 9. pp. 5469-5475.
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