Abstract
In this study, we characterized the molecular events involved in the activation of the ubiquitous transcription factor NF-κB by the viral transactivator pX. pX expression in HeLa cells determines a manyfold increase in NF-κB-dependent transcription, which is associated with an increase in p50/p65 heterodimer DNA-binding activity. Since the IκB-α inhibitory subunit proteolytic degradation, which follows its phosphorylation/modification, is a key event in NF-κB activation by different stimuli (such as growth factors, phorbol esters, tumor necrosis factor, UV irradiation, and oxygen radicals), we investigated pX effects on IκB-α, as well as the possible involvement of known signalling pathways in pX-induced NF-κB-dependent transcription. We observed that although pX had no direct effect on p50 or p65, it was able to restore the IκB-α-suppressed p50/p65 activity. More directly, the stable expression of pX in HeLa cells resulted in reduced levels of IκB-α in the cytoplasm. Pretreatment of the cells with H7, calphostin C, tyrphostin 25, or N-acetylcysteine did not impair the effects of pX on NF-κB, thus ruling out the involvement of protein kinase C, tyrosine kinases, and oxygen radicals. Finally, while most of the known NF-κB-activating agents converge on Raf-1 protein kinase, when Raf-1 activity is blocked by overexpression of a dominant negative mutant, the effects of pX on NF-κB are not impaired. Thus, we suggest that although pX is able to activate the Ras/Raf-1-signalling pathway, it triggers NF-κB activation by an as yet unidentified Raf-1-independent pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 641-646 |
| Number of pages | 6 |
| Journal | Journal of Virology |
| Volume | 70 |
| Issue number | 1 |
| Publication status | Published - 1996 |
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ASJC Scopus subject areas
- Immunology
Cite this
Hepatitis B virus pX activates NF-κB-dependent transcription through a raf-independent pathway. / Chirillo, Paolo; Falco, Mirella; Puri, Pier Lorenzo; Artini, Marco; Balsano, Clara; Levrero, Massimo; Natoli, Gioacchino.
In: Journal of Virology, Vol. 70, No. 1, 1996, p. 641-646.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatitis B virus pX activates NF-κB-dependent transcription through a raf-independent pathway
AU - Chirillo, Paolo
AU - Falco, Mirella
AU - Puri, Pier Lorenzo
AU - Artini, Marco
AU - Balsano, Clara
AU - Levrero, Massimo
AU - Natoli, Gioacchino
PY - 1996
Y1 - 1996
N2 - In this study, we characterized the molecular events involved in the activation of the ubiquitous transcription factor NF-κB by the viral transactivator pX. pX expression in HeLa cells determines a manyfold increase in NF-κB-dependent transcription, which is associated with an increase in p50/p65 heterodimer DNA-binding activity. Since the IκB-α inhibitory subunit proteolytic degradation, which follows its phosphorylation/modification, is a key event in NF-κB activation by different stimuli (such as growth factors, phorbol esters, tumor necrosis factor, UV irradiation, and oxygen radicals), we investigated pX effects on IκB-α, as well as the possible involvement of known signalling pathways in pX-induced NF-κB-dependent transcription. We observed that although pX had no direct effect on p50 or p65, it was able to restore the IκB-α-suppressed p50/p65 activity. More directly, the stable expression of pX in HeLa cells resulted in reduced levels of IκB-α in the cytoplasm. Pretreatment of the cells with H7, calphostin C, tyrphostin 25, or N-acetylcysteine did not impair the effects of pX on NF-κB, thus ruling out the involvement of protein kinase C, tyrosine kinases, and oxygen radicals. Finally, while most of the known NF-κB-activating agents converge on Raf-1 protein kinase, when Raf-1 activity is blocked by overexpression of a dominant negative mutant, the effects of pX on NF-κB are not impaired. Thus, we suggest that although pX is able to activate the Ras/Raf-1-signalling pathway, it triggers NF-κB activation by an as yet unidentified Raf-1-independent pathway.
AB - In this study, we characterized the molecular events involved in the activation of the ubiquitous transcription factor NF-κB by the viral transactivator pX. pX expression in HeLa cells determines a manyfold increase in NF-κB-dependent transcription, which is associated with an increase in p50/p65 heterodimer DNA-binding activity. Since the IκB-α inhibitory subunit proteolytic degradation, which follows its phosphorylation/modification, is a key event in NF-κB activation by different stimuli (such as growth factors, phorbol esters, tumor necrosis factor, UV irradiation, and oxygen radicals), we investigated pX effects on IκB-α, as well as the possible involvement of known signalling pathways in pX-induced NF-κB-dependent transcription. We observed that although pX had no direct effect on p50 or p65, it was able to restore the IκB-α-suppressed p50/p65 activity. More directly, the stable expression of pX in HeLa cells resulted in reduced levels of IκB-α in the cytoplasm. Pretreatment of the cells with H7, calphostin C, tyrphostin 25, or N-acetylcysteine did not impair the effects of pX on NF-κB, thus ruling out the involvement of protein kinase C, tyrosine kinases, and oxygen radicals. Finally, while most of the known NF-κB-activating agents converge on Raf-1 protein kinase, when Raf-1 activity is blocked by overexpression of a dominant negative mutant, the effects of pX on NF-κB are not impaired. Thus, we suggest that although pX is able to activate the Ras/Raf-1-signalling pathway, it triggers NF-κB activation by an as yet unidentified Raf-1-independent pathway.
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M3 - Article
VL - 70
SP - 641
EP - 646
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 1
ER -