Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection

Francesco Leone; Herve' Zylberberg; Giovanni Squadrito; Bernard Le Guen; Pierre Berthelot; Stanislas Pol; Christian Brechot

doi:10.1016/S0168-8278(98)80247-X

Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection

Francesco Leone, Herve' Zylberberg, Giovanni Squadrito, Bernard Le Guen, Pierre Berthelot, Stanislas Pol, Christian Brechot

Istituto Oncologico Candiolo

Research output: Contribution to journal › Article › peer-review

Abstract

Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI

Original language	English
Pages (from-to)	689-694
Number of pages	6
Journal	Journal of Hepatology
Volume	29
Issue number	5
DOIs	https://doi.org/10.1016/S0168-8278(98)80247-X
Publication status	Published - Nov 1998

Keywords

HCV quasispecies
Knodell's grading
PCR-SSCP analysis

ASJC Scopus subject areas

Gastroenterology

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10.1016/S0168-8278(98)80247-X

Cite this

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title = "Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection",

abstract = "Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI ",

keywords = "HCV quasispecies, Knodell's grading, PCR-SSCP analysis",

author = "Francesco Leone and Herve' Zylberberg and Giovanni Squadrito and {Le Guen}, Bernard and Pierre Berthelot and Stanislas Pol and Christian Brechot",

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T1 - Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection

AU - Leone, Francesco

AU - Zylberberg, Herve'

AU - Squadrito, Giovanni

AU - Le Guen, Bernard

AU - Berthelot, Pierre

AU - Pol, Stanislas

AU - Brechot, Christian

PY - 1998/11

Y1 - 1998/11

N2 - Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI

AB - Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI

KW - HCV quasispecies

KW - Knodell's grading

KW - PCR-SSCP analysis

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Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection

Abstract

Keywords

ASJC Scopus subject areas

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