Abstract
Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI
| Original language | English |
|---|---|
| Pages (from-to) | 689-694 |
| Number of pages | 6 |
| Journal | Journal of Hepatology |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 1998 |
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Keywords
- HCV quasispecies
- Knodell's grading
- PCR-SSCP analysis
ASJC Scopus subject areas
- Gastroenterology
Cite this
Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection. / Leone, Francesco; Zylberberg, Herve'; Squadrito, Giovanni; Le Guen, Bernard; Berthelot, Pierre; Pol, Stanislas; Brechot, Christian.
In: Journal of Hepatology, Vol. 29, No. 5, 11.1998, p. 689-694.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatitis C virus (HCV) hypervariable region 1 complexity does not correlate with severity of liver disease, HCV type, viral load or duration of infection
AU - Leone, Francesco
AU - Zylberberg, Herve'
AU - Squadrito, Giovanni
AU - Le Guen, Bernard
AU - Berthelot, Pierre
AU - Pol, Stanislas
AU - Brechot, Christian
PY - 1998/11
Y1 - 1998/11
N2 - Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI
AB - Background/Aims: Several studies have suggested that infection with a highly heterogeneous population of hepatitis C virus correlates with a low response rate to interferon. It is still debated, however, whether or not this heterogeneity might be associated with liver disease severity. The aim of this study was to analyse hepatitis C virus genome complexity in patients with various stages of liver disease. Methods: We used polymerase chain reaction-single strand conformation polymorphism (SSCP) analysis to estimate the degree of complexity of the hypervariable region (HVR1) in 95 anti-HCV and serum HCV-RNA positive patients. They were divided into two groups according to Knodell's histological activity index (HAI) grading: 22 with HAI
KW - HCV quasispecies
KW - Knodell's grading
KW - PCR-SSCP analysis
UR - http://www.scopus.com/inward/record.url?scp=16744364557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16744364557&partnerID=8YFLogxK
U2 - 10.1016/S0168-8278(98)80247-X
DO - 10.1016/S0168-8278(98)80247-X
M3 - Article
C2 - 9833904
AN - SCOPUS:16744364557
VL - 29
SP - 689
EP - 694
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 5
ER -