Hepatitis C virus: life cycle in cells, infection and host response, and analysis of molecular markers influencing the outcome of infection and response to therapy

L. B. Dustin, Barbara Bartolini, M. R. Capobianchi, M. Pistello

Research output: Contribution to journalReview article

Abstract

Hepatitis C virus (HCV) is a major global health burden accounting for around 170 million chronic infections worldwide. Since its discovery, which dates back to about 30 years ago, many details of the viral genome organization and the astonishing genetic diversity have been unveiled but, owing to the difficulty of culturing HCV in vitro and obtaining fully susceptible yet immunocompetent in vivo models, we are still a long way from the full comprehension of viral life cycle, host cell pathways facilitating or counteracting infection, pathogenetic mechanisms in vivo, and host defences. Here, we illustrate the viral life cycle into cells, describe the interplay between immune and genetic host factors shaping the course of infection, and provide details of the molecular approaches currently used to genotype, monitor replication in vivo, and study the emergence of drug-resistant viral variants.

Original languageEnglish
Pages (from-to)826-832
Number of pages7
JournalClinical Microbiology and Infection
Volume22
Issue number10
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Life Cycle Stages
Hepacivirus
Infection
Viral Genome
Therapeutics
Genotype
Pharmaceutical Preparations

Keywords

  • Broadly reactive neutralizing antibodies
  • Cell culture-derived HCV
  • Direct antiviral agents
  • Drug resistance
  • Genotype
  • Hepatitis C virus
  • Host response
  • monitoring drug resistance
  • Pathogenesis
  • Quantitative molecular assays

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

@article{07c3de8abfe0489a821d674c53e9aa2e,
title = "Hepatitis C virus: life cycle in cells, infection and host response, and analysis of molecular markers influencing the outcome of infection and response to therapy",
abstract = "Hepatitis C virus (HCV) is a major global health burden accounting for around 170 million chronic infections worldwide. Since its discovery, which dates back to about 30 years ago, many details of the viral genome organization and the astonishing genetic diversity have been unveiled but, owing to the difficulty of culturing HCV in vitro and obtaining fully susceptible yet immunocompetent in vivo models, we are still a long way from the full comprehension of viral life cycle, host cell pathways facilitating or counteracting infection, pathogenetic mechanisms in vivo, and host defences. Here, we illustrate the viral life cycle into cells, describe the interplay between immune and genetic host factors shaping the course of infection, and provide details of the molecular approaches currently used to genotype, monitor replication in vivo, and study the emergence of drug-resistant viral variants.",
keywords = "Broadly reactive neutralizing antibodies, Cell culture-derived HCV, Direct antiviral agents, Drug resistance, Genotype, Hepatitis C virus, Host response, monitoring drug resistance, Pathogenesis, Quantitative molecular assays",
author = "Dustin, {L. B.} and Barbara Bartolini and Capobianchi, {M. R.} and M. Pistello",
year = "2016",
month = "10",
day = "1",
doi = "10.1016/j.cmi.2016.08.025",
language = "English",
volume = "22",
pages = "826--832",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier B.V.",
number = "10",

}

TY - JOUR

T1 - Hepatitis C virus

T2 - life cycle in cells, infection and host response, and analysis of molecular markers influencing the outcome of infection and response to therapy

AU - Dustin, L. B.

AU - Bartolini, Barbara

AU - Capobianchi, M. R.

AU - Pistello, M.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Hepatitis C virus (HCV) is a major global health burden accounting for around 170 million chronic infections worldwide. Since its discovery, which dates back to about 30 years ago, many details of the viral genome organization and the astonishing genetic diversity have been unveiled but, owing to the difficulty of culturing HCV in vitro and obtaining fully susceptible yet immunocompetent in vivo models, we are still a long way from the full comprehension of viral life cycle, host cell pathways facilitating or counteracting infection, pathogenetic mechanisms in vivo, and host defences. Here, we illustrate the viral life cycle into cells, describe the interplay between immune and genetic host factors shaping the course of infection, and provide details of the molecular approaches currently used to genotype, monitor replication in vivo, and study the emergence of drug-resistant viral variants.

AB - Hepatitis C virus (HCV) is a major global health burden accounting for around 170 million chronic infections worldwide. Since its discovery, which dates back to about 30 years ago, many details of the viral genome organization and the astonishing genetic diversity have been unveiled but, owing to the difficulty of culturing HCV in vitro and obtaining fully susceptible yet immunocompetent in vivo models, we are still a long way from the full comprehension of viral life cycle, host cell pathways facilitating or counteracting infection, pathogenetic mechanisms in vivo, and host defences. Here, we illustrate the viral life cycle into cells, describe the interplay between immune and genetic host factors shaping the course of infection, and provide details of the molecular approaches currently used to genotype, monitor replication in vivo, and study the emergence of drug-resistant viral variants.

KW - Broadly reactive neutralizing antibodies

KW - Cell culture-derived HCV

KW - Direct antiviral agents

KW - Drug resistance

KW - Genotype

KW - Hepatitis C virus

KW - Host response

KW - monitoring drug resistance

KW - Pathogenesis

KW - Quantitative molecular assays

UR - http://www.scopus.com/inward/record.url?scp=84995700058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84995700058&partnerID=8YFLogxK

U2 - 10.1016/j.cmi.2016.08.025

DO - 10.1016/j.cmi.2016.08.025

M3 - Review article

AN - SCOPUS:84995700058

VL - 22

SP - 826

EP - 832

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 10

ER -