Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013–2015

Roberto Bruni, Umbertina Villano, Michele Equestre, Paola Chionne, Elisabetta Madonna, Dilyana Trandeva-Bankova, Maria Peleva-Pishmisheva, Tencho Tenev, Eleonora Cella, Massimo Ciccozzi, Giulio Pisani, Elitsa Golkocheva-Markova, Anna Rita Ciccaglione

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

Background In industrialized areas of the world, including Europe, Hepatitis E Virus (HEV) is considered an emerging pathogen. In fact, autochthonous cases caused by HEV genotype 3 (HEV-3) are increasingly reported. Several studies described the human HEV-3 subtypes and strains circulating in West Europe countries; in contrast, very little is known about the HEV strains responsible for acute hepatitis E in countries of East Europe/Balkans, such as Bulgaria. Methods and findings Anti-HEV IgM positive serum samples (n = 103) from acute hepatitis cases (2013–2015) from all over Bulgaria were analysed for HEV RNA by Real-Time PCR. Viremia was detected in 90/ 103 samples. A fragment of the viral genome (ORF-2 region) was amplified by nested PCR from 76/90 viremic samples, leading to a sequence in 64 of them. Genotyping by phylogenetic analysis with standard reference sequences showed HEV-1 in 1/64 cases, HEV-3 in 63/64. Subtyping of HEV-3 sequences showed 3e (39/63, 62%), 3f (n = 15/63, 24%) and 3c (n = 8/63, 13%) subtypes; in one case the sequence subtype was uncertain and classified as 3hi. In the phylogenetic tree, most 3e sequences grouped in two well distinct clusters (A and B), each one with very low intragroup genetic distances. In contrast, 3f and 3c were interspersed with reference sequences and showed lower tendency to cluster and/or higher intragroup distances. Geographically, while 3f and 3c were scattered throughout the country, 3e was restricted to the South-West area, with most cases in two towns about 40 kilometres apart from each other. Conclusions Most acute hepatitis E cases in Bulgaria are caused by HEV-3, subtypes 3e, 3f and 3c. Circulation of 3e appears quite different from 3f and 3c, with 3e restricted to the South-West area while 3f and 3c diffused over the country. The factors underlying the observed molecular and geographical differences remain to be investigated.

Original languageEnglish
Article numbere0198045
JournalPLoS One
Volume13
Issue number6
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Hepatitis E virus
Bulgaria
hepatitis
Viruses
Hepatitis
Genotype
genotype
hepatitis E
Hepatitis E
Balkan Peninsula
Viral Genome
Balkans
Viremia
viremia
reference standards
phylogeny
Pathogens
sampling
towns
genotyping

Keywords

  • hepatitis E virus
  • Bulgaria
  • Epidemiology
  • Phylogeny

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013–2015. / Bruni, Roberto; Villano, Umbertina; Equestre, Michele; Chionne, Paola; Madonna, Elisabetta; Trandeva-Bankova, Dilyana; Peleva-Pishmisheva, Maria; Tenev, Tencho; Cella, Eleonora; Ciccozzi, Massimo; Pisani, Giulio; Golkocheva-Markova, Elitsa; Ciccaglione, Anna Rita.

In: PLoS One, Vol. 13, No. 6, e0198045, 01.06.2018.

Research output: Contribution to journalReview article

Bruni, R, Villano, U, Equestre, M, Chionne, P, Madonna, E, Trandeva-Bankova, D, Peleva-Pishmisheva, M, Tenev, T, Cella, E, Ciccozzi, M, Pisani, G, Golkocheva-Markova, E & Ciccaglione, AR 2018, 'Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013–2015', PLoS One, vol. 13, no. 6, e0198045. https://doi.org/10.1371/journal.pone.0198045
Bruni, Roberto ; Villano, Umbertina ; Equestre, Michele ; Chionne, Paola ; Madonna, Elisabetta ; Trandeva-Bankova, Dilyana ; Peleva-Pishmisheva, Maria ; Tenev, Tencho ; Cella, Eleonora ; Ciccozzi, Massimo ; Pisani, Giulio ; Golkocheva-Markova, Elitsa ; Ciccaglione, Anna Rita. / Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013–2015. In: PLoS One. 2018 ; Vol. 13, No. 6.
@article{ef4b419f71764880bc4afdf3c8895a00,
title = "Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013–2015",
abstract = "Background In industrialized areas of the world, including Europe, Hepatitis E Virus (HEV) is considered an emerging pathogen. In fact, autochthonous cases caused by HEV genotype 3 (HEV-3) are increasingly reported. Several studies described the human HEV-3 subtypes and strains circulating in West Europe countries; in contrast, very little is known about the HEV strains responsible for acute hepatitis E in countries of East Europe/Balkans, such as Bulgaria. Methods and findings Anti-HEV IgM positive serum samples (n = 103) from acute hepatitis cases (2013–2015) from all over Bulgaria were analysed for HEV RNA by Real-Time PCR. Viremia was detected in 90/ 103 samples. A fragment of the viral genome (ORF-2 region) was amplified by nested PCR from 76/90 viremic samples, leading to a sequence in 64 of them. Genotyping by phylogenetic analysis with standard reference sequences showed HEV-1 in 1/64 cases, HEV-3 in 63/64. Subtyping of HEV-3 sequences showed 3e (39/63, 62{\%}), 3f (n = 15/63, 24{\%}) and 3c (n = 8/63, 13{\%}) subtypes; in one case the sequence subtype was uncertain and classified as 3hi. In the phylogenetic tree, most 3e sequences grouped in two well distinct clusters (A and B), each one with very low intragroup genetic distances. In contrast, 3f and 3c were interspersed with reference sequences and showed lower tendency to cluster and/or higher intragroup distances. Geographically, while 3f and 3c were scattered throughout the country, 3e was restricted to the South-West area, with most cases in two towns about 40 kilometres apart from each other. Conclusions Most acute hepatitis E cases in Bulgaria are caused by HEV-3, subtypes 3e, 3f and 3c. Circulation of 3e appears quite different from 3f and 3c, with 3e restricted to the South-West area while 3f and 3c diffused over the country. The factors underlying the observed molecular and geographical differences remain to be investigated.",
keywords = "hepatitis E virus, Bulgaria, Epidemiology, Phylogeny",
author = "Roberto Bruni and Umbertina Villano and Michele Equestre and Paola Chionne and Elisabetta Madonna and Dilyana Trandeva-Bankova and Maria Peleva-Pishmisheva and Tencho Tenev and Eleonora Cella and Massimo Ciccozzi and Giulio Pisani and Elitsa Golkocheva-Markova and Ciccaglione, {Anna Rita}",
year = "2018",
month = "6",
day = "1",
doi = "10.1371/journal.pone.0198045",
language = "English",
volume = "13",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Hepatitis E virus genotypes and subgenotypes causing acute hepatitis, Bulgaria, 2013–2015

AU - Bruni, Roberto

AU - Villano, Umbertina

AU - Equestre, Michele

AU - Chionne, Paola

AU - Madonna, Elisabetta

AU - Trandeva-Bankova, Dilyana

AU - Peleva-Pishmisheva, Maria

AU - Tenev, Tencho

AU - Cella, Eleonora

AU - Ciccozzi, Massimo

AU - Pisani, Giulio

AU - Golkocheva-Markova, Elitsa

AU - Ciccaglione, Anna Rita

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Background In industrialized areas of the world, including Europe, Hepatitis E Virus (HEV) is considered an emerging pathogen. In fact, autochthonous cases caused by HEV genotype 3 (HEV-3) are increasingly reported. Several studies described the human HEV-3 subtypes and strains circulating in West Europe countries; in contrast, very little is known about the HEV strains responsible for acute hepatitis E in countries of East Europe/Balkans, such as Bulgaria. Methods and findings Anti-HEV IgM positive serum samples (n = 103) from acute hepatitis cases (2013–2015) from all over Bulgaria were analysed for HEV RNA by Real-Time PCR. Viremia was detected in 90/ 103 samples. A fragment of the viral genome (ORF-2 region) was amplified by nested PCR from 76/90 viremic samples, leading to a sequence in 64 of them. Genotyping by phylogenetic analysis with standard reference sequences showed HEV-1 in 1/64 cases, HEV-3 in 63/64. Subtyping of HEV-3 sequences showed 3e (39/63, 62%), 3f (n = 15/63, 24%) and 3c (n = 8/63, 13%) subtypes; in one case the sequence subtype was uncertain and classified as 3hi. In the phylogenetic tree, most 3e sequences grouped in two well distinct clusters (A and B), each one with very low intragroup genetic distances. In contrast, 3f and 3c were interspersed with reference sequences and showed lower tendency to cluster and/or higher intragroup distances. Geographically, while 3f and 3c were scattered throughout the country, 3e was restricted to the South-West area, with most cases in two towns about 40 kilometres apart from each other. Conclusions Most acute hepatitis E cases in Bulgaria are caused by HEV-3, subtypes 3e, 3f and 3c. Circulation of 3e appears quite different from 3f and 3c, with 3e restricted to the South-West area while 3f and 3c diffused over the country. The factors underlying the observed molecular and geographical differences remain to be investigated.

AB - Background In industrialized areas of the world, including Europe, Hepatitis E Virus (HEV) is considered an emerging pathogen. In fact, autochthonous cases caused by HEV genotype 3 (HEV-3) are increasingly reported. Several studies described the human HEV-3 subtypes and strains circulating in West Europe countries; in contrast, very little is known about the HEV strains responsible for acute hepatitis E in countries of East Europe/Balkans, such as Bulgaria. Methods and findings Anti-HEV IgM positive serum samples (n = 103) from acute hepatitis cases (2013–2015) from all over Bulgaria were analysed for HEV RNA by Real-Time PCR. Viremia was detected in 90/ 103 samples. A fragment of the viral genome (ORF-2 region) was amplified by nested PCR from 76/90 viremic samples, leading to a sequence in 64 of them. Genotyping by phylogenetic analysis with standard reference sequences showed HEV-1 in 1/64 cases, HEV-3 in 63/64. Subtyping of HEV-3 sequences showed 3e (39/63, 62%), 3f (n = 15/63, 24%) and 3c (n = 8/63, 13%) subtypes; in one case the sequence subtype was uncertain and classified as 3hi. In the phylogenetic tree, most 3e sequences grouped in two well distinct clusters (A and B), each one with very low intragroup genetic distances. In contrast, 3f and 3c were interspersed with reference sequences and showed lower tendency to cluster and/or higher intragroup distances. Geographically, while 3f and 3c were scattered throughout the country, 3e was restricted to the South-West area, with most cases in two towns about 40 kilometres apart from each other. Conclusions Most acute hepatitis E cases in Bulgaria are caused by HEV-3, subtypes 3e, 3f and 3c. Circulation of 3e appears quite different from 3f and 3c, with 3e restricted to the South-West area while 3f and 3c diffused over the country. The factors underlying the observed molecular and geographical differences remain to be investigated.

KW - hepatitis E virus

KW - Bulgaria

KW - Epidemiology

KW - Phylogeny

UR - http://www.scopus.com/inward/record.url?scp=85048171199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048171199&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0198045

DO - 10.1371/journal.pone.0198045

M3 - Review article

C2 - 29879148

AN - SCOPUS:85048171199

VL - 13

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e0198045

ER -