Hepatocyte autotransplantation into spleen using cirrhotic rats

F. Calise, E. Mezza, A. Ceriello, A. Mancini, G. Pettinato, A. Scognamiglio, D. Scala, S. Cozzolino

Research output: Contribution to journalArticle

Abstract

Syngeneic rat hepatocytes transplanted into different sites such as spleen, peritoneum, lung and pancreas have proved to proliferate and provide some liver functions to correct congenital liver disorders or acute liver failure. In a previous series in which hepatocytes from Lewis rats were allotransplanted into cirrhotic Wistar-Furth rats, we showed that the most effective immunosuppressive dosage is 12 mg/kg cyclosporine A three times a week. However, it is yet to be demonstrated whether hepatocytes can be kept longer than 180 days, and 50% hepatectomy is always required. In this study, the feasibility of autotransplantation of hepatocytes in cirrhotic Wistar rats is evaluated. After viability was determined by trypan blue exclusion, 5 x 106 hepatocytes were injected. Rats were sacrificed and their spleens removed between 7 and 180 days after autotransplantation, at which time they were assessed on the basis of periodic acid-schiff reaction and levels of cytokeratin A1-A3, cytokeratin 19 and technetium 99 uptake. A good Tc-99 uptake was observed, but there was no evidence of either complete bile ductular-like structures or significantly improved laboratory findings. Our study has shown that autotransplantation is feasible and is associated with long-term hepatocyte survival. However, as confirmed by the experiences of Mite and coworkers in humans, the fate of hepatocytes after six months remains uncertain, and the harvesting of hepatocytes from severely cirrhotic livers entails a series of problems.

Original languageEnglish
Pages (from-to)141-143
Number of pages3
JournalTransplantology: Journal of Cell and Organ Transplantation
Volume5
Issue number4
Publication statusPublished - 1994

Fingerprint

Autologous Transplantation
Hepatocytes
Spleen
Liver
Inbred WF Rats
Periodic Acid-Schiff Reaction
Keratin-19
Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Trypan Blue
Acute Liver Failure
Mites
Peritoneum
Technetium
Hepatectomy
Immunosuppressive Agents
Keratins
Bile
Cyclosporine
Wistar Rats
Pancreas

Keywords

  • Autotransplantation
  • Cirrhosis
  • Hepatocytes

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Calise, F., Mezza, E., Ceriello, A., Mancini, A., Pettinato, G., Scognamiglio, A., ... Cozzolino, S. (1994). Hepatocyte autotransplantation into spleen using cirrhotic rats. Transplantology: Journal of Cell and Organ Transplantation, 5(4), 141-143.

Hepatocyte autotransplantation into spleen using cirrhotic rats. / Calise, F.; Mezza, E.; Ceriello, A.; Mancini, A.; Pettinato, G.; Scognamiglio, A.; Scala, D.; Cozzolino, S.

In: Transplantology: Journal of Cell and Organ Transplantation, Vol. 5, No. 4, 1994, p. 141-143.

Research output: Contribution to journalArticle

Calise, F, Mezza, E, Ceriello, A, Mancini, A, Pettinato, G, Scognamiglio, A, Scala, D & Cozzolino, S 1994, 'Hepatocyte autotransplantation into spleen using cirrhotic rats', Transplantology: Journal of Cell and Organ Transplantation, vol. 5, no. 4, pp. 141-143.
Calise, F. ; Mezza, E. ; Ceriello, A. ; Mancini, A. ; Pettinato, G. ; Scognamiglio, A. ; Scala, D. ; Cozzolino, S. / Hepatocyte autotransplantation into spleen using cirrhotic rats. In: Transplantology: Journal of Cell and Organ Transplantation. 1994 ; Vol. 5, No. 4. pp. 141-143.
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