Hepatocyte growth factor/scatter factor released during peritonitis is active on mesothelial cells

T. Rampino, G. Cancarini, M. Gregorini, P. Guallini, M. Maggio, A. Ranghino, G. Soccio, A. Dal Canton

Research output: Contribution to journalArticle

Abstract

Peritonitis causes mesothelial detachment that may result in persistent peritoneal denudation and fibrosis. We investigated whether hepatocyte growth factor (HGF), a scatter factor that induces detachment from substrate and fibroblastic transformation of several cell types, is produced during peritonitis and is active on mesothelial cells. We studied 18 patients on peritoneal dialysis, 9 uncomplicated, 9 with peritonitis. HGF was measured in serum, peritoneal fluid, and supernatant of peripheral blood mononuclear cells and peritoneal mononuclear cells. Primary culture of human peritoneal mesothelial cells and the human mesothelial cell line MeT-5A were conditioned with recombinant HGF, serum, and peritoneal fluid. HGF levels were significantly higher in serum and peritoneal fluid of peritonitic than uncomplicated patients. Mononuclear cells of peritonitic patients produced more HGF than cells of uncomplicated patients. Recombinant HGF, serum, and peritoneal fluid of peritonitic patients caused mesothelial cell growth, detachment, transformation from epithelial to fibroblast-like shape, overexpression of vimentin, and synthesis of type I and III collagen. In conclusion, HGF released during peritonitis causes a change in mesothelial cell phenotype and function. HGF may affect the healing process facilitating repair through mesothelial cell growth, but may contribute to peritoneal fibrosis inducing cell detachment with mesothelial denudation and collagen synthesis.

Original languageEnglish
Pages (from-to)1275-1285
Number of pages11
JournalAmerican Journal of Pathology
Volume159
Issue number4
Publication statusPublished - 2001

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Hepatocyte Growth Factor
Peritonitis
Ascitic Fluid
Peritoneal Fibrosis
Serum
Collagen Type III
Peritoneal Dialysis
Vimentin
Growth
Collagen Type I
Blood Cells
Collagen
Fibroblasts
Phenotype
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Rampino, T., Cancarini, G., Gregorini, M., Guallini, P., Maggio, M., Ranghino, A., ... Dal Canton, A. (2001). Hepatocyte growth factor/scatter factor released during peritonitis is active on mesothelial cells. American Journal of Pathology, 159(4), 1275-1285.

Hepatocyte growth factor/scatter factor released during peritonitis is active on mesothelial cells. / Rampino, T.; Cancarini, G.; Gregorini, M.; Guallini, P.; Maggio, M.; Ranghino, A.; Soccio, G.; Dal Canton, A.

In: American Journal of Pathology, Vol. 159, No. 4, 2001, p. 1275-1285.

Research output: Contribution to journalArticle

Rampino, T, Cancarini, G, Gregorini, M, Guallini, P, Maggio, M, Ranghino, A, Soccio, G & Dal Canton, A 2001, 'Hepatocyte growth factor/scatter factor released during peritonitis is active on mesothelial cells', American Journal of Pathology, vol. 159, no. 4, pp. 1275-1285.
Rampino, T. ; Cancarini, G. ; Gregorini, M. ; Guallini, P. ; Maggio, M. ; Ranghino, A. ; Soccio, G. ; Dal Canton, A. / Hepatocyte growth factor/scatter factor released during peritonitis is active on mesothelial cells. In: American Journal of Pathology. 2001 ; Vol. 159, No. 4. pp. 1275-1285.
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