TY - JOUR
T1 - HER3 genomic gain and sensitivity to gefitinib in advanced non-small-cell lung cancer patients
AU - Cappuzzo, F.
AU - Toschi, L.
AU - Domenichini, I.
AU - Bartolini, S.
AU - Ceresoli, G. L.
AU - Rossi, E.
AU - Ludovini, V.
AU - Cancellieri, A.
AU - Magrini, E.
AU - Bemis, L.
AU - Franklin, W. A.
AU - Crino, L.
AU - Bunn, P. A.
AU - Hirsch, F. R.
AU - Varella-Garcia, M.
PY - 2005/12/12
Y1 - 2005/12/12
N2 - In non-small-cell lung cancer (NSCLC), sensitivity to tyrosine kinase inhibitors (TKIs) is associated with activating mutations and genomic gain of the epidermal growth factor receptor (EGFR). Preclinical data suggested that HER3 overexpression increases sensitivity to TKIs. A total of 82 NSCLC patients treated with gefitinib (250 mg), and previously evaluated for EGFR and HER2 status by fluorescence in situ hybridisation (FISH) and DNA sequencing, and for Phospho-Akt status by immunohistochemistry, were investigated for HER3 genomic gain by FISH. Patients with high polysomy and gene amplification were considered as HER3 FISH positive (+). HER3 FISH + pattern was significantly associated with female gender (P = 0.02) and never smoking history (P = 0.02). Patients with HER3+ tumours (26.8%) had a significantly longer time to progression (3.7 vs 2.7, P = 0.04) than patients with HER3-tumours, but not a significantly better response rate or survival. Patients with EGFR+/HER3+ tumours had higher objective response rate (36.4 vs 9.9%, P = 0.03) and time to progression (7.7 vs 2.7 months, P = 0.03) than patients with EGFR- and/or HER3- tumours, but no significantly longer survival. No difference in response was observed according to HER3 status in patients with EGFR+ tumours. Patients with HER2+/HER3+ tumours had similar outcome as patients with HER2- and/or HER3-tumours. Significantly different clinical end points were not observed between patients with HER3+/P-Akt+ and HER3- and/or P-Akt- tumours. Genomic gain for HER3 is not a marker for response or resistance to TKI therapy in advanced NSCLC patients.
AB - In non-small-cell lung cancer (NSCLC), sensitivity to tyrosine kinase inhibitors (TKIs) is associated with activating mutations and genomic gain of the epidermal growth factor receptor (EGFR). Preclinical data suggested that HER3 overexpression increases sensitivity to TKIs. A total of 82 NSCLC patients treated with gefitinib (250 mg), and previously evaluated for EGFR and HER2 status by fluorescence in situ hybridisation (FISH) and DNA sequencing, and for Phospho-Akt status by immunohistochemistry, were investigated for HER3 genomic gain by FISH. Patients with high polysomy and gene amplification were considered as HER3 FISH positive (+). HER3 FISH + pattern was significantly associated with female gender (P = 0.02) and never smoking history (P = 0.02). Patients with HER3+ tumours (26.8%) had a significantly longer time to progression (3.7 vs 2.7, P = 0.04) than patients with HER3-tumours, but not a significantly better response rate or survival. Patients with EGFR+/HER3+ tumours had higher objective response rate (36.4 vs 9.9%, P = 0.03) and time to progression (7.7 vs 2.7 months, P = 0.03) than patients with EGFR- and/or HER3- tumours, but no significantly longer survival. No difference in response was observed according to HER3 status in patients with EGFR+ tumours. Patients with HER2+/HER3+ tumours had similar outcome as patients with HER2- and/or HER3-tumours. Significantly different clinical end points were not observed between patients with HER3+/P-Akt+ and HER3- and/or P-Akt- tumours. Genomic gain for HER3 is not a marker for response or resistance to TKI therapy in advanced NSCLC patients.
KW - EGFR
KW - Gefitinib
KW - HER3
KW - Non-small-cell lung cancer
KW - Tyrosine kinase inhibitor
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U2 - 10.1038/sj.bjc.6602865
DO - 10.1038/sj.bjc.6602865
M3 - Article
C2 - 16288303
AN - SCOPUS:28644444702
VL - 93
SP - 1334
EP - 1340
JO - British Journal of Cancer
JF - British Journal of Cancer
SN - 0007-0920
IS - 12
ER -