HER/erbB receptors as therapeutic targets of immunotoxins in human rhabdomyosarcoma cells

Cinzia Ricci, Letizia Polito, Patrizia Nanni, Lorena Landuzzi, Annalisa Astolfi, Giordano Nicoletti, Ilaria Rossi, Carla De Giovanni, Andrea Bolognesi, Pier Luigi Lollini

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Human rhabdomyosarcoma cells express HER/erbB growth factors receptors. Receptors belonging to this family are overexpressed and play a role in many types of epithelial and neural cancer and have been selected as targets for cancer therapy. In this paper EGF-R, HER-2 and HER-3 receptors were tested as therapeutic targets of immunotoxins in human rhabdomyosarcoma. Rhabdomyosarcoma cells were treated with indirect immunotoxins consisting in primary specific murine monoclonal antibodies recognizing EGF-R, HER-2 and HER-3 followed by secondary F(ab′)2 antimouse immunoglobulin linked to saporin-S6, a type 1 ribosome-inactivating protein (RIP) from the seeds of Saponaria officinalis. The indirect immunotoxin targeting EGF-R caused a significant inhibition in cell growth and protein synthesis and a strong increase in apoptosis in rhabdomyosarcoma cells, whereas indirect immunotoxins against HER-2 and HER-3 were ineffective. The toxic activity of anti-EGF-R immunotoxin was also observed on rhabdomyosarcoma cells expressing low level of EGF-R. EGF-R could be a novel therapeutic target of immunotoxins in human rhabdomyosarcoma.

Original languageEnglish
Pages (from-to)314-323
Number of pages10
JournalJournal of Immunotherapy
Volume25
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

Immunotoxins
Rhabdomyosarcoma
Epidermal Growth Factor
Type 1 Ribosome Inactivating Proteins
Saponaria
Therapeutics
S 6
Growth Factor Receptors
Poisons
ErbB Receptors
Immunoglobulins
Neoplasms
Seeds
Monoclonal Antibodies
Apoptosis
Growth
Proteins

Keywords

  • Apoptosis
  • HER/ErbB receptors
  • Immunotoxins
  • Protein synthesis
  • Rhabdomyosarcoma

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Pharmacology

Cite this

HER/erbB receptors as therapeutic targets of immunotoxins in human rhabdomyosarcoma cells. / Ricci, Cinzia; Polito, Letizia; Nanni, Patrizia; Landuzzi, Lorena; Astolfi, Annalisa; Nicoletti, Giordano; Rossi, Ilaria; De Giovanni, Carla; Bolognesi, Andrea; Lollini, Pier Luigi.

In: Journal of Immunotherapy, Vol. 25, No. 4, 2002, p. 314-323.

Research output: Contribution to journalArticle

Ricci, C, Polito, L, Nanni, P, Landuzzi, L, Astolfi, A, Nicoletti, G, Rossi, I, De Giovanni, C, Bolognesi, A & Lollini, PL 2002, 'HER/erbB receptors as therapeutic targets of immunotoxins in human rhabdomyosarcoma cells', Journal of Immunotherapy, vol. 25, no. 4, pp. 314-323. https://doi.org/10.1097/00002371-200207000-00003
Ricci, Cinzia ; Polito, Letizia ; Nanni, Patrizia ; Landuzzi, Lorena ; Astolfi, Annalisa ; Nicoletti, Giordano ; Rossi, Ilaria ; De Giovanni, Carla ; Bolognesi, Andrea ; Lollini, Pier Luigi. / HER/erbB receptors as therapeutic targets of immunotoxins in human rhabdomyosarcoma cells. In: Journal of Immunotherapy. 2002 ; Vol. 25, No. 4. pp. 314-323.
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