Herpes simplex virus type-specific antibody determination by enzyme-linked immunosorbent assay in human sera

Maria Grazia Revello, Giuseppe Gerna

Research output: Contribution to journalArticlepeer-review

Abstract

Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42%) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalSerodiagnosis and Immunotherapy in Infectious Disease
Volume2
Issue number6
DOIs
Publication statusPublished - 1988

Keywords

  • ELISA
  • HSV antibody typing
  • HSV strain typing
  • microneutralization

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)

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