Herpes simplex virus type-specific antibody determination by enzyme-linked immunosorbent assay in human sera

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Abstract

Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42%) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.

Original languageEnglish
Pages (from-to)415-422
Number of pages8
JournalSerodiagnosis and Immunotherapy in Infectious Disease
Volume2
Issue number6
DOIs
Publication statusPublished - 1988

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Human Herpesvirus 2
Simplexvirus
Human Herpesvirus 1
Enzyme-Linked Immunosorbent Assay
Antibodies
Serum
Antigens
Viral Antigens
Virus Diseases
Immunoglobulin G

Keywords

  • ELISA
  • HSV antibody typing
  • HSV strain typing
  • microneutralization

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)

Cite this

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title = "Herpes simplex virus type-specific antibody determination by enzyme-linked immunosorbent assay in human sera",
abstract = "Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42{\%}) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.",
keywords = "ELISA, HSV antibody typing, HSV strain typing, microneutralization",
author = "Revello, {Maria Grazia} and Giuseppe Gerna",
year = "1988",
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N2 - Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42%) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.

AB - Herpes simplex virus (HSV) type-specific IgG antibody was determined by an indirect enzyme-linked immunosorbent assay (ELISA) which requires optimal concentrations of HSV-1 and HSV-2 crude antigens absorbed to the solid phase and the calculation of the ratio (r) between absorbance values obtained with HSV-1 and HSV-2 antigens. Reference pools of human sera containing HSV-1 or HSV-2 type-specific antibody, identified by microneutralization, were used to calibrate the optimal concentrations of HSV-1 and HSV-2 antigens, which were selected on the basis of the highest differential reactivity with the homotypic as compared to the heterotypic viral antigen. r value ranges for HSV-1- (>2·00) and HSV-2- ( 2.00 (mean 2.9H) whereas in the HSV-2 group of patients, 21 (42%) were showp to have HSV-2 type-specific antibody only (rs between 1·50 and 2·00 and one > 2·00. Anamnestic data available from five of these seven patients indicated a relatively recent (1-6 months) primary HSV-2 infection in the presence of remote HSV-1 antibody.

KW - ELISA

KW - HSV antibody typing

KW - HSV strain typing

KW - microneutralization

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