Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression

Inès Martìn-Padura, Roberta Mortarini, Davide Lauri, Sergio Bernasconi, Francisco Sanchez-Madrid, Giorgio Parmiani, Alberto Mantovani, Andrea Anichini, Elisabetta Dejana

Research output: Contribution to journalArticle

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Abstract

Tumor cell attachment to endothelial cells (EC) is one of the critical steps of the metastatic process. It was previously reported that interleukin 1 treatment of EC induces expression of membrane molecules that promote tumor cell adhesion. In this paper we report that a panel of six clones isolated from a human metastatic melanoma presented a marked heterogeneity in their ability to adhere to interleukin 1 activated EC. This was correlated with integrin VLA-4 expression by the clones. Antibodies directed to VLA-4 and to its endothelial ligand INCAM110/ VCAM-1 abolished interleukin 1 induced increase in melanoma cell adhesion to EC. These data demonstrate intratumor heterogeneity in the expression of VLA-4 and that this can represent a crucial determinant of tumor cell interaction with EC during secondary spread.

Original languageEnglish
Pages (from-to)2239-2241
Number of pages3
JournalCancer Research
Volume51
Issue number8
Publication statusPublished - Apr 15 1991

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Integrin alpha4beta1
Cell Adhesion
Melanoma
Endothelial Cells
Cytokines
Interleukin-1
Clone Cells
Neoplasms
Vascular Cell Adhesion Molecule-1
Integrins
Cell Communication
Ligands
Membranes
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Martìn-Padura, I., Mortarini, R., Lauri, D., Bernasconi, S., Sanchez-Madrid, F., Parmiani, G., ... Dejana, E. (1991). Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression. Cancer Research, 51(8), 2239-2241.

Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression. / Martìn-Padura, Inès; Mortarini, Roberta; Lauri, Davide; Bernasconi, Sergio; Sanchez-Madrid, Francisco; Parmiani, Giorgio; Mantovani, Alberto; Anichini, Andrea; Dejana, Elisabetta.

In: Cancer Research, Vol. 51, No. 8, 15.04.1991, p. 2239-2241.

Research output: Contribution to journalArticle

Martìn-Padura, I, Mortarini, R, Lauri, D, Bernasconi, S, Sanchez-Madrid, F, Parmiani, G, Mantovani, A, Anichini, A & Dejana, E 1991, 'Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression', Cancer Research, vol. 51, no. 8, pp. 2239-2241.
Martìn-Padura I, Mortarini R, Lauri D, Bernasconi S, Sanchez-Madrid F, Parmiani G et al. Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression. Cancer Research. 1991 Apr 15;51(8):2239-2241.
Martìn-Padura, Inès ; Mortarini, Roberta ; Lauri, Davide ; Bernasconi, Sergio ; Sanchez-Madrid, Francisco ; Parmiani, Giorgio ; Mantovani, Alberto ; Anichini, Andrea ; Dejana, Elisabetta. / Heterogeneity in human melanoma cell adhesion to cytokine activated endothelial cells correlates with VLA-4 expression. In: Cancer Research. 1991 ; Vol. 51, No. 8. pp. 2239-2241.
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