Heterogeneity in the expression of HLA and tumor-associated antigens by surgically removed and cultured breast carcinoma cells

P. G. Natali, P. Giacomini, A. Bigotti, K. Imai, M. R. Nicotra, A. K. Ng, S. Ferrone

Research output: Contribution to journalArticle

Abstract

Surgically removed normal and malignant mammary tissues and human breast carcinoma cell lines were tested in binding assays with monoclonal antibodies to HLA-A,B,C antigens, β 2-microglobulin, HLA-DR antigens, and tumor-associated antigens; the latter included a Mr 280,000, a Mr 94,000, and a Mr 85,000 membrane-bound glycoprotein and a cytoplasmic antigen. HLA-A,B antigens, β 2-microglobulin, HLA-DR antigens, and the cytoplasmic antigen are expressed by normal mammary cells. Their malignant transformation may be associated with quantitative changes in the expression of these antigens and with the appearance of Mr 94,000 and Mr 85,000 glycoproteins. The Mr 280,000 glycoprotein was detected on only one of the breast carcinoma cell lines tested. Analysis of primary tumors and autologous axillary lymph node metastasis from 13 patients has shown differences in the expression of all the antigens tested between primary and metastatic lesions.

Original languageEnglish
Pages (from-to)660-668
Number of pages9
JournalCancer Research
Volume43
Issue number2
Publication statusPublished - 1983

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Natali, P. G., Giacomini, P., Bigotti, A., Imai, K., Nicotra, M. R., Ng, A. K., & Ferrone, S. (1983). Heterogeneity in the expression of HLA and tumor-associated antigens by surgically removed and cultured breast carcinoma cells. Cancer Research, 43(2), 660-668.