Heterogeneity of B cell growth factor (BCGF)-producing T cells in humans. Clonal analysis of BCGF-producing cells within T4+ and T8+ subsets and evidence for the involvement of different growth factors in different BCGF assays

Maria Cristina Mingari, Franca Gerosa, Enrico Maggi, Raffaella Cozzani, Daniela Pende, Cristina Bottino, Alessandro Poggi, Sergio Romagnani, Lorenzo Moretta

Research output: Contribution to journalArticlepeer-review

Abstract

Human peripheral blood T cells were cloned under conditions allowing the clonal expansion of virtually all T cells. Clones derived from T4+ or T4-(T8+) subsets were screened for their ability to induce B cell proliferation either in the Staphylococcus aureus Cowan-I (SAC)-driven assay or in the costimulation assay based on the use of anti-μ antibodies. BCGF activity in the SAC-and in the anti-μ-driven system was displayed by 13% and 15% T8+ clones, respectively, while 74% and 79% T4+ clones had BCGF activity in the same assay. SN of clones with BCGF activity were further screened for their susceptibility to the inhibition by CM 269 (anti-IL-2 receptor) monoclonal antibody. All clones were inhibited in the SAC assay, whereas several clones were partially or totally resistant to inhibition in the anti-μ assay. Nine of such non-inhibited clones were further analyzed for their ability to produce γ-IFN (known to act as a BCGF) and IL-2: six of them were found to produce γ-IFN, while none produced any detectable IL-2 activity.

Original languageEnglish
Pages (from-to)23-28
Number of pages6
JournalLa Ricerca in Clinica e in Laboratorio
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 1986

Keywords

  • γ-interferon
  • B cell growth factor
  • Interleukin-2
  • Monoclonal antibodies
  • T cell clones
  • T lymphocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

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