Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver

George W. Cox, Bonnie J. Mathieson, Lucia Gandino, Elisabetta Blasi, Danuta Radzioch, Luigi Varesio

Research output: Contribution to journalArticlepeer-review

Abstract

The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macro-phages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leuko-cytic on the basis of its expression of the Ly-6B.2, FcγR, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions. [J Natl Cancer Inst 81: 1492-1496, 1989]

Original languageEnglish
Pages (from-to)1492-1496
Number of pages5
JournalJournal of the National Cancer Institute
Volume81
Issue number19
DOIs
Publication statusPublished - Apr 4 1989

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Oncology
  • Cancer Research

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