Abstract
The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macro-phages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leuko-cytic on the basis of its expression of the Ly-6B.2, FcγR, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions. [J Natl Cancer Inst 81: 1492-1496, 1989]
Original language | English |
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Pages (from-to) | 1492-1496 |
Number of pages | 5 |
Journal | Journal of the National Cancer Institute |
Volume | 81 |
Issue number | 19 |
DOIs | |
Publication status | Published - Apr 4 1989 |
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Physiology (medical)
- Radiology Nuclear Medicine and imaging
- Oncology
- Cancer Research