Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver

George W. Cox; Bonnie J. Mathieson; Lucia Gandino; Elisabetta Blasi; Danuta Radzioch; Luigi Varesio

doi:10.1093/jnci/81.19.1492

Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver

George W. Cox, Bonnie J. Mathieson, Lucia Gandino, Elisabetta Blasi, Danuta Radzioch, Luigi Varesio

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macro-phages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5^a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leuko-cytic on the basis of its expression of the Ly-6B.2, FcγR, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions. [J Natl Cancer Inst 81: 1492-1496, 1989]

Original language	English
Pages (from-to)	1492-1496
Number of pages	5
Journal	Journal of the National Cancer Institute
Volume	81
Issue number	19
DOIs	https://doi.org/10.1093/jnci/81.19.1492
Publication status	Published - Apr 4 1989

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Physiology (medical)
Radiology Nuclear Medicine and imaging
Oncology
Cancer Research

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Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver. / Cox, George W.; Mathieson, Bonnie J.; Gandino, Lucia; Blasi, Elisabetta; Radzioch, Danuta; Varesio, Luigi.

In: Journal of the National Cancer Institute, Vol. 81, No. 19, 04.04.1989, p. 1492-1496.

Research output: Contribution to journal › Article › peer-review

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title = "Heterogeneity of hematopoietic cells immortalized by v-myc/v-raf recombinant retrovirus infection of bone marrow or fetal liver",

abstract = "The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macro-phages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leuko-cytic on the basis of its expression of the Ly-6B.2, FcγR, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions. [J Natl Cancer Inst 81: 1492-1496, 1989]",

author = "Cox, {George W.} and Mathieson, {Bonnie J.} and Lucia Gandino and Elisabetta Blasi and Danuta Radzioch and Luigi Varesio",

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AU - Cox, George W.

AU - Mathieson, Bonnie J.

AU - Gandino, Lucia

AU - Blasi, Elisabetta

AU - Radzioch, Danuta

AU - Varesio, Luigi

PY - 1989/4/4

Y1 - 1989/4/4

N2 - The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macro-phages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leuko-cytic on the basis of its expression of the Ly-6B.2, FcγR, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions. [J Natl Cancer Inst 81: 1492-1496, 1989]

AB - The J2 recombinant retrovirus expressing v-myc/v-raf (also known as MYC/RAF1) immortalized macro-phages from the bone marrow of lipopolysaccharide-responsive mouse strains, producing the ANA-1 cell line from C57BL/6 mice and the INF-3A cell line from C3H/HeN mice. In contrast, J2 recombinant retrovirus infection of the fetal liver from C57BL/6-Ly-5a mice immortalized a cell line (GGD) that did not exhibit the characteristics of mature macrophages. The GGD cell line was classified as leuko-cytic on the basis of its expression of the Ly-6B.2, FcγR, and Ly-5.2 antigens. Our results indicate that the J2 recombinant retrovirus selectively immortalizes macrophages from the bone marrow of C57BL/6 and C3H/HeN mice but immortalizes cells without definitive macrophage characteristics from murine fetal liver under the same culture conditions. [J Natl Cancer Inst 81: 1492-1496, 1989]

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