Heterogeneity of large cell carcinoma of the lung: An immunophenotypic and miRNA-based analysis

Mattia Barbareschi; Chiara Cantaloni; Valerio Del Vescovo; Alberto Cavazza; Valentina Monica; Rodolfo Carella; Giulio Rossi; Luca Morelli; Alberto Cucino; Massimo Silvestri; Giuseppe Tirone; Giuseppe Pelosi; Paolo Graziano; Mauro Papotti; Paolo Dalla Palma; Claudio Doglioni; Michela Alessandra Denti

doi:10.1309/AJCPYY79XAGRAYCJ

Heterogeneity of large cell carcinoma of the lung: An immunophenotypic and miRNA-based analysis

Mattia Barbareschi, Chiara Cantaloni, Valerio Del Vescovo, Alberto Cavazza, Valentina Monica, Rodolfo Carella, Giulio Rossi, Luca Morelli, Alberto Cucino, Massimo Silvestri, Giuseppe Tirone, Giuseppe Pelosi, Paolo Graziano, Mauro Papotti, Paolo Dalla Palma, Claudio Doglioni, Michela Alessandra Denti

Research output: Contribution to journal › Article

Abstract

Large cell carcinomas (LCCs) of the lung are heterogeneous and may be of different cell lineages. We analyzed 56 surgically resected lung tumors classified as LCC on the basis of pure morphologic grounds, using a panel of immunophenotypic markers (adenocarcinoma [ADC]-specific, thyroid transcription factor-1, cytokeratin 7, and napsin A; squamous cell carcinoma [SQCC]-specific, p63, cytokeratin 5, desmocollin 3, and Δnp63) and the quantitative analysis of microRNA-205 (microRNA sample score [mRSS]). Based on immunoprofiles 19 (34%) of the cases were reclassified as ADC and 14 (25%) as SQCC; 23 (41%) of the cases were unclassifiable. Of these 23 cases, 18 were classified as ADC and 5 as SQCC according to the mRSS. Our data show that an extended panel of immunohistochemical markers can reclassify around 60% of LCCs as ADC or SQCC. However, a relevant percentage of LCCs may escape convincing immunohistochemical classification, and mRSS could be used for further typing, but its clinical relevance needs further confirmation.

Original language	English
Pages (from-to)	773-782
Number of pages	10
Journal	American Journal of Clinical Pathology
Volume	136
Issue number	5
DOIs	https://doi.org/10.1309/AJCPYY79XAGRAYCJ
Publication status	Published - Nov 2011

Keywords

Adenocarcinoma
Heterogeneity
Immunophenotype
Large cell carcinoma
Lung neoplasms
miRNA
Squamous cell carcinoma

ASJC Scopus subject areas

Pathology and Forensic Medicine

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Barbareschi, M., Cantaloni, C., Vescovo, V. D., Cavazza, A., Monica, V., Carella, R., Rossi, G., Morelli, L., Cucino, A., Silvestri, M., Tirone, G., Pelosi, G., Graziano, P., Papotti, M., Palma, P. D., Doglioni, C., & Denti, M. A. (2011). Heterogeneity of large cell carcinoma of the lung: An immunophenotypic and miRNA-based analysis. American Journal of Clinical Pathology, 136(5), 773-782. https://doi.org/10.1309/AJCPYY79XAGRAYCJ

Heterogeneity of large cell carcinoma of the lung : An immunophenotypic and miRNA-based analysis. / Barbareschi, Mattia; Cantaloni, Chiara; Vescovo, Valerio Del; Cavazza, Alberto; Monica, Valentina; Carella, Rodolfo; Rossi, Giulio; Morelli, Luca; Cucino, Alberto; Silvestri, Massimo; Tirone, Giuseppe; Pelosi, Giuseppe ; Graziano, Paolo; Papotti, Mauro; Palma, Paolo Dalla; Doglioni, Claudio; Denti, Michela Alessandra.

In: American Journal of Clinical Pathology, Vol. 136, No. 5, 11.2011, p. 773-782.

Research output: Contribution to journal › Article

Barbareschi, M, Cantaloni, C, Vescovo, VD, Cavazza, A, Monica, V, Carella, R, Rossi, G, Morelli, L, Cucino, A, Silvestri, M, Tirone, G, Pelosi, G , Graziano, P, Papotti, M, Palma, PD, Doglioni, C & Denti, MA 2011, 'Heterogeneity of large cell carcinoma of the lung: An immunophenotypic and miRNA-based analysis', American Journal of Clinical Pathology, vol. 136, no. 5, pp. 773-782. https://doi.org/10.1309/AJCPYY79XAGRAYCJ

Barbareschi, Mattia ; Cantaloni, Chiara ; Vescovo, Valerio Del ; Cavazza, Alberto ; Monica, Valentina ; Carella, Rodolfo ; Rossi, Giulio ; Morelli, Luca ; Cucino, Alberto ; Silvestri, Massimo ; Tirone, Giuseppe ; Pelosi, Giuseppe ; Graziano, Paolo ; Papotti, Mauro ; Palma, Paolo Dalla ; Doglioni, Claudio ; Denti, Michela Alessandra. / Heterogeneity of large cell carcinoma of the lung : An immunophenotypic and miRNA-based analysis. In: American Journal of Clinical Pathology. 2011 ; Vol. 136, No. 5. pp. 773-782.

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abstract = "Large cell carcinomas (LCCs) of the lung are heterogeneous and may be of different cell lineages. We analyzed 56 surgically resected lung tumors classified as LCC on the basis of pure morphologic grounds, using a panel of immunophenotypic markers (adenocarcinoma [ADC]-specific, thyroid transcription factor-1, cytokeratin 7, and napsin A; squamous cell carcinoma [SQCC]-specific, p63, cytokeratin 5, desmocollin 3, and Δnp63) and the quantitative analysis of microRNA-205 (microRNA sample score [mRSS]). Based on immunoprofiles 19 (34%) of the cases were reclassified as ADC and 14 (25%) as SQCC; 23 (41%) of the cases were unclassifiable. Of these 23 cases, 18 were classified as ADC and 5 as SQCC according to the mRSS. Our data show that an extended panel of immunohistochemical markers can reclassify around 60% of LCCs as ADC or SQCC. However, a relevant percentage of LCCs may escape convincing immunohistochemical classification, and mRSS could be used for further typing, but its clinical relevance needs further confirmation.",

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