Abstract
Large cell carcinomas (LCCs) of the lung are heterogeneous and may be of different cell lineages. We analyzed 56 surgically resected lung tumors classified as LCC on the basis of pure morphologic grounds, using a panel of immunophenotypic markers (adenocarcinoma [ADC]-specific, thyroid transcription factor-1, cytokeratin 7, and napsin A; squamous cell carcinoma [SQCC]-specific, p63, cytokeratin 5, desmocollin 3, and Δnp63) and the quantitative analysis of microRNA-205 (microRNA sample score [mRSS]). Based on immunoprofiles 19 (34%) of the cases were reclassified as ADC and 14 (25%) as SQCC; 23 (41%) of the cases were unclassifiable. Of these 23 cases, 18 were classified as ADC and 5 as SQCC according to the mRSS. Our data show that an extended panel of immunohistochemical markers can reclassify around 60% of LCCs as ADC or SQCC. However, a relevant percentage of LCCs may escape convincing immunohistochemical classification, and mRSS could be used for further typing, but its clinical relevance needs further confirmation.
| Original language | English |
|---|---|
| Pages (from-to) | 773-782 |
| Number of pages | 10 |
| Journal | American Journal of Clinical Pathology |
| Volume | 136 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 2011 |
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Keywords
- Adenocarcinoma
- Heterogeneity
- Immunophenotype
- Large cell carcinoma
- Lung neoplasms
- miRNA
- Squamous cell carcinoma
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Heterogeneity of large cell carcinoma of the lung : An immunophenotypic and miRNA-based analysis. / Barbareschi, Mattia; Cantaloni, Chiara; Vescovo, Valerio Del; Cavazza, Alberto; Monica, Valentina; Carella, Rodolfo; Rossi, Giulio; Morelli, Luca; Cucino, Alberto; Silvestri, Massimo; Tirone, Giuseppe; Pelosi, Giuseppe; Graziano, Paolo; Papotti, Mauro; Palma, Paolo Dalla; Doglioni, Claudio; Denti, Michela Alessandra.
In: American Journal of Clinical Pathology, Vol. 136, No. 5, 11.2011, p. 773-782.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Heterogeneity of large cell carcinoma of the lung
T2 - An immunophenotypic and miRNA-based analysis
AU - Barbareschi, Mattia
AU - Cantaloni, Chiara
AU - Vescovo, Valerio Del
AU - Cavazza, Alberto
AU - Monica, Valentina
AU - Carella, Rodolfo
AU - Rossi, Giulio
AU - Morelli, Luca
AU - Cucino, Alberto
AU - Silvestri, Massimo
AU - Tirone, Giuseppe
AU - Pelosi, Giuseppe
AU - Graziano, Paolo
AU - Papotti, Mauro
AU - Palma, Paolo Dalla
AU - Doglioni, Claudio
AU - Denti, Michela Alessandra
PY - 2011/11
Y1 - 2011/11
N2 - Large cell carcinomas (LCCs) of the lung are heterogeneous and may be of different cell lineages. We analyzed 56 surgically resected lung tumors classified as LCC on the basis of pure morphologic grounds, using a panel of immunophenotypic markers (adenocarcinoma [ADC]-specific, thyroid transcription factor-1, cytokeratin 7, and napsin A; squamous cell carcinoma [SQCC]-specific, p63, cytokeratin 5, desmocollin 3, and Δnp63) and the quantitative analysis of microRNA-205 (microRNA sample score [mRSS]). Based on immunoprofiles 19 (34%) of the cases were reclassified as ADC and 14 (25%) as SQCC; 23 (41%) of the cases were unclassifiable. Of these 23 cases, 18 were classified as ADC and 5 as SQCC according to the mRSS. Our data show that an extended panel of immunohistochemical markers can reclassify around 60% of LCCs as ADC or SQCC. However, a relevant percentage of LCCs may escape convincing immunohistochemical classification, and mRSS could be used for further typing, but its clinical relevance needs further confirmation.
AB - Large cell carcinomas (LCCs) of the lung are heterogeneous and may be of different cell lineages. We analyzed 56 surgically resected lung tumors classified as LCC on the basis of pure morphologic grounds, using a panel of immunophenotypic markers (adenocarcinoma [ADC]-specific, thyroid transcription factor-1, cytokeratin 7, and napsin A; squamous cell carcinoma [SQCC]-specific, p63, cytokeratin 5, desmocollin 3, and Δnp63) and the quantitative analysis of microRNA-205 (microRNA sample score [mRSS]). Based on immunoprofiles 19 (34%) of the cases were reclassified as ADC and 14 (25%) as SQCC; 23 (41%) of the cases were unclassifiable. Of these 23 cases, 18 were classified as ADC and 5 as SQCC according to the mRSS. Our data show that an extended panel of immunohistochemical markers can reclassify around 60% of LCCs as ADC or SQCC. However, a relevant percentage of LCCs may escape convincing immunohistochemical classification, and mRSS could be used for further typing, but its clinical relevance needs further confirmation.
KW - Adenocarcinoma
KW - Heterogeneity
KW - Immunophenotype
KW - Large cell carcinoma
KW - Lung neoplasms
KW - miRNA
KW - Squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=80355129921&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80355129921&partnerID=8YFLogxK
U2 - 10.1309/AJCPYY79XAGRAYCJ
DO - 10.1309/AJCPYY79XAGRAYCJ
M3 - Article
C2 - 22031317
AN - SCOPUS:80355129921
VL - 136
SP - 773
EP - 782
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 5
ER -