Heterogeneity of NGF-induced differentiation in PC12 cells investigated in a battery of isolated cell clones

E. Clementi, M. Raichman, J. Meldolesi

Research output: Contribution to journalArticlepeer-review

Abstract

The effect of NGF on a set of 17 PC12 cell clones was investigated in order to clarify some aspects concerning the changes in cell morphology and Ca2+ homeostasis that occur in the PC12 cell line after NGF-induced differentiation. The morphological parameters analyzed showed a high degree of heterogeneity among clones, consisting with a different expression of the high-affintiy NGF receptor. The NGF effect on Ca2+ influx was investigated in a clone highly sensitive to the cholinergic agonist carbachol. Results showed an increase in Ca2+ influx through one of the carbachol-activated Ca2+ channels.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalFunctional Neurology
Volume8
Issue number2
Publication statusPublished - 1993

Keywords

  • Ca homeostasis
  • carbachol
  • clones
  • differentiation
  • NGF
  • PC12 cells

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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