Heterogeneity of the α-interferon-mediated overexpression of class-I and class-II major histocompatibility complex molecules in primary cultured cancer cells

C. Semino, G. Ferlazzo, G. Pietra, W. Pasquetti, L. Repetto, R. Rosso, M. Mariani, G. Melioli

Research output: Contribution to journalArticlepeer-review

Abstract

Human α- and γ-interferons (IFNs) have the capability of enhancing the expression of major histocompatibility complex-class I and class II (MHC-I and MHC-II) as well as other surface tumor associated antigens (TAA) on cancer cell lines. This capability, associated to the anti-proliferative effect observed both in vitro and in vivo, was the basis of the extended use of IFN in cancer patients. Despite the great expectations, several solid neoplasms resulted resistant to treatment with IFNs. In order to analyze the actual effects of IFNs on cancer, we treated primary cultures of different histotypes of cancer cell, which are well known to differ significantly from established cell lines, with escalating doses of α-IFN. In this paper, we demonstrate that a variable proportion of culture was able to potentiate the expression of MHC-I and MHC-II molecules on the surface. This finding suggests that a clear functional heterogeneity was present in primary cultures of cancer cells, that strictly reflected the in vivo situation. In addition, it is of note that in some histotypes, 'natural' lymphoblastoid α-IFN was more effective than recombinant human α-IFN in the induction of this capability.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalJournal of Biological Regulators and Homeostatic Agents
Volume7
Issue number3
Publication statusPublished - 1993

Keywords

  • α-IFN
  • cancer cells
  • heterogeneity
  • MHC-I
  • MHC-II

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Endocrinology
  • Physiology
  • Immunology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Physiology (medical)

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