Heterologous in vivo processing of human preproendothelin 1 into bioactive peptides

Maria Serena Fabbrini, Alessandro Vitale, Carlo Patrono, Moreno Zamai, Fabrizio Vaghi, Valeria Caiolfa, Lucia Monaco, Luca Benatti

Research output: Contribution to journalArticle

Abstract

Endothelin (ET) is an extremely potent vasoconstrictor peptide of 21 amino acids, originally found in the supernatant of cultured vascular endothelial cells. To gain insights into its biosynthetic pathway, we expressed a synthetic RNA coding for the 212-amino acid precursor of human ET-1 (preproET-1) in Xenopus oocytes. Cell homogenates and oocyte incubation medium were tested by RIA using an anti-ET-1 serum. ET-1-like immunoreactivity was detected in oocytes injected with preproET-1 synthetic RNA but not in control oocytes and was much higher in medium than in cell homogenates. When preproET-1 was expressed in oocytes treated with monensin, a dramatic decrease in secretion of immunoreactive material was observed, indicating that secretion is mediated by the Golgi complex. ET-1-like immunoreactive material present in oocyte incubation medium was fractionated by reverse-phase HPLC into two main peaks, corresponding to the retention times of human big ET-1 and ET-1. Incubation medium of oocytes expressing the synthetic preproET-1 RNA elicited a characteristic vasoconstrictor response on rabbit vena cava, consistent with the biological activity that would be predicted from the amount of ET-1-like immunoreactivity measured. These results suggest that common pathways of ET maturation exist in widely different cells and that Xenopus oocytes may represent a useful tool in studying the cell biology of ET-1 synthesis.

Original languageEnglish
Pages (from-to)8939-8943
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number20
Publication statusPublished - Oct 15 1991

Fingerprint

Endothelin-1
Oocytes
Peptides
Endothelins
Vasoconstrictor Agents
RNA
Xenopus
Amino Acids
Monensin
Venae Cavae
Biosynthetic Pathways
Golgi Apparatus
Cell Biology
Endothelial Cells
High Pressure Liquid Chromatography
Rabbits
Serum

Keywords

  • Endothelin
  • Protein secretion
  • Vasoconstriction
  • Xenopus oocytes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Heterologous in vivo processing of human preproendothelin 1 into bioactive peptides. / Fabbrini, Maria Serena; Vitale, Alessandro; Patrono, Carlo; Zamai, Moreno; Vaghi, Fabrizio; Caiolfa, Valeria; Monaco, Lucia; Benatti, Luca.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 20, 15.10.1991, p. 8939-8943.

Research output: Contribution to journalArticle

Fabbrini, MS, Vitale, A, Patrono, C, Zamai, M, Vaghi, F, Caiolfa, V, Monaco, L & Benatti, L 1991, 'Heterologous in vivo processing of human preproendothelin 1 into bioactive peptides', Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 20, pp. 8939-8943.
Fabbrini, Maria Serena ; Vitale, Alessandro ; Patrono, Carlo ; Zamai, Moreno ; Vaghi, Fabrizio ; Caiolfa, Valeria ; Monaco, Lucia ; Benatti, Luca. / Heterologous in vivo processing of human preproendothelin 1 into bioactive peptides. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 20. pp. 8939-8943.
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