Il trapianto eterotopico del cuore di ratto: una metodica sperimentale per la valutazione della protezione miocardica.

Translated title of the contribution: Heterotopic heart transplantation in the rat: an experimental method for evaluating myocardial protection

A. M. Perna, A. Borgioli, M. Bonacchi, C. Varini, G. Vaccari

Research output: Contribution to journalArticlepeer-review

Abstract

This research represents the first part of a project finalized to prolong the ischemic time of the explanted heart. Our experimental model utilizes rat hearts heterotopically transplanted into the abdomen of a recipient: a denervated heart but perfused by blood as to permit a good evaluation of the reperfusion damage. We used 3 different types of cardioplegic solutions to achieve cardiac arrest: the first of the intracellular fluid type, the second of the extracellular type and the third one as a standard cardioplegic solution supplemented by Ca++ antagonist. Cold saline 4 degrees C was used as control. Histological patterns, birefringence index and intracellular content of high energy phosphates were evaluated. Specimens were taken after 1 hour of ischemic time and after 1 hour of reperfusion. Early results show that administration of cardioplegic solution maintains birefringence index and preserve high energy phosphates: among the 3 different types of solution the intracellular type seems to achieve better results although the number of experiments is too small to show any statistical significance. These data show that this model is appropriate to test cardioplegic solutions and their effectiveness.

Translated title of the contributionHeterotopic heart transplantation in the rat: an experimental method for evaluating myocardial protection
Original languageItalian
Pages (from-to)721-724
Number of pages4
JournalCardiologia
Volume37
Issue number10
Publication statusPublished - Oct 1992

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Heterotopic heart transplantation in the rat: an experimental method for evaluating myocardial protection'. Together they form a unique fingerprint.

Cite this