Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions

Laura Pirovano; Simone Culurgioni; Manuel Carminati; Andrea Alfieri; Silvia Monzani; Valentina Cecatiello; Chiara Gaddoni; Francesca Rizzelli; James Foadi; Sebastiano Pasqualato; Marina Mapelli

doi:10.1038/s41467-019-09999-w

Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions

Laura Pirovano, Simone Culurgioni, Manuel Carminati, Andrea Alfieri, Silvia Monzani, Valentina Cecatiello, Chiara Gaddoni, Francesca Rizzelli, James Foadi, Sebastiano Pasqualato, Marina Mapelli

Istituto Europeo di Oncologia

Research output: Contribution to journal › Article › peer-review

Abstract

Cortical force generators connect epithelial polarity sites with astral microtubules, allowing dynein movement to orient the mitotic spindle as astral microtubules depolymerize. Complexes of the LGN and NuMA proteins, fundamental components of force generators, are recruited to the cortex by Gαi-subunits of heterotrimeric G-proteins. They associate with dynein/dynactin and activate the motor activity pulling on astral microtubules. The architecture of cortical force generators is unknown. Here we report the crystal structure of NuMA:LGN hetero-hexamers, and unveil their role in promoting the assembly of active cortical dynein/dynactin motors that are required in orchestrating oriented divisions in polarized cells. Our work elucidates the basis for the structural organization of essential spindle orientation motors.

Original language	English
Article number	2208
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-09999-w
Publication status	Published - Dec 1 2019

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/s41467-019-09999-w

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Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions. / Pirovano, Laura; Culurgioni, Simone; Carminati, Manuel; Alfieri, Andrea; Monzani, Silvia ; Cecatiello, Valentina ; Gaddoni, Chiara ; Rizzelli, Francesca; Foadi, James; Pasqualato, Sebastiano ; Mapelli, Marina.

In: Nature Communications, Vol. 10, No. 1, 2208, 01.12.2019.

Research output: Contribution to journal › Article › peer-review

Pirovano, Laura ; Culurgioni, Simone ; Carminati, Manuel ; Alfieri, Andrea ; Monzani, Silvia ; Cecatiello, Valentina ; Gaddoni, Chiara ; Rizzelli, Francesca ; Foadi, James ; Pasqualato, Sebastiano ; Mapelli, Marina. / Hexameric NuMA:LGN structures promote multivalent interactions required for planar epithelial divisions. In: Nature Communications. 2019 ; Vol. 10, No. 1.

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AU - Cecatiello, Valentina

AU - Gaddoni, Chiara

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