HHV-6 infects human aortic and heart microvascular endothelial cells, increasing their ability to secrete proinflammatory chemokines

Arnaldo Caruso, Antonella Rotola, Manola Comar, Flavia Favilli, Monica Galvan, Maria Tosetti, Cesare Campello, Elisabetta Caselli, Giulio Alessandri, Manuela Grassi, Emirena Garrafa, Enzo Cassai, Dario Di Luca

Research output: Contribution to journalArticle

Abstract

Endothelial cells are important targets for herpesvirus infection. To evaluate the biological effects of human herpesvirus-6 (HHV-6) infection, adult heart microvascular and aortic endothelial cells were examined for in vitro susceptibility to HHV-6 and for the alterations induced by viral infection on the production of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8). Analysis by reverse transcription-polymerase chain reaction and by in situ polymerase chain reaction showed that HHV-6 replicates in endothelium in the absence of cytopathic effects, and that viral sequences were present in 20% umbilical vein and in 10% aortic and 1% microvascular endothelium. HHV-6 infection upregulated the production of MCP-1 and IL-8, with differences observed between aortic and microvascular endothelium. These findings demonstrate that endothelial cells represent a potential reservoir for HHV-6 infection, and the altered pattern of chemokine production can lead to attraction of immunocompetent cells and to the development of inflammatory processes.

Original languageEnglish
Pages (from-to)528-533
Number of pages6
JournalJournal of Medical Virology
Volume67
Issue number4
DOIs
Publication statusPublished - 2002

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Keywords

  • Endothelium
  • Herpesvirus
  • IL-8
  • Inflammation
  • MCP-1

ASJC Scopus subject areas

  • Virology

Cite this

Caruso, A., Rotola, A., Comar, M., Favilli, F., Galvan, M., Tosetti, M., Campello, C., Caselli, E., Alessandri, G., Grassi, M., Garrafa, E., Cassai, E., & Di Luca, D. (2002). HHV-6 infects human aortic and heart microvascular endothelial cells, increasing their ability to secrete proinflammatory chemokines. Journal of Medical Virology, 67(4), 528-533. https://doi.org/10.1002/jmv.10133