HHV-8 DNA replication correlates with the clinical status in AIDS-related Kaposi's sarcoma

Francesco Broccolo, Chiara Tassan Din, Maria Grazia Viganò, Teresa Rutigliano, Susanna Esposito, P. Lusso, Giuseppe Tambussi, Mauro Malnati

Research output: Contribution to journalArticle

Abstract

Background: The value of plasma levels of human herpesvirus 8 (HHV-8) DNA as a marker of clinical status in acquired immunodeficiency syndrome-related Kaposi's sarcoma (AIDS-KS) remains to be elucidated. Objectives: To investigate the relationship between the plasma HHV-8 DNA viral load and the clinical status of AIDS-KS. Study Design: A total of 378 blood samples were obtained from 62 patients with AIDS-KS followed longitudinally. All patients received antiretroviral therapy (ART) or anti-neoplastic therapy. The patients were divided into four groups according to their clinical status: onset disease (OD), progressive disease (PD), stable or partial remission (S/PR) and complete remission (CR). Results: Plasma HHV-8 DNAaemia was detected in all samples obtained from patients with OD or PD (100%); in contrast, HHV-8 DNAaemia was found only in a minority of patients with CR (8%) and was invariably undetectable in patients with stable CR. HHV-8 DNA detection in plasma was strongly associated with an unfavourable outcome (odds ratio = 231.9; p <0.0001). Conversely, neither the HIV-1 viral load nor peripheral CD4+ T-cell counts were associated with the KS clinical status, though both parameters did affect HHV-8 DNAaemia levels (p <0.0001). Multivariate analysis confirmed that HHV-8 DNAaemia was strongly and independently correlated with both clinical status (p <0.05) and HIV-1 plasma viraemia (p = 0.027). Conclusions: The strong association of plasma HHV-8 DNAaemia with onset or progressive disease is compatible with an active role of replicating virus in clinically active AIDS-KS. An accurate evaluation of the plasma HHV-8 load might be useful for monitoring AIDS-KS under antiretroviral or antineoplastic therapy.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Clinical Virology
Volume78
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Human Herpesvirus 8
DNA Replication
Kaposi's Sarcoma
Acquired Immunodeficiency Syndrome
Viral Load
HIV-1
DNA
AIDS-related Kaposi sarcoma
Viremia
CD4 Lymphocyte Count
Antineoplastic Agents
Therapeutics
Multivariate Analysis
Biomarkers
Odds Ratio
Viruses
T-Lymphocytes

Keywords

  • Disease progression
  • Human herpesvirus-8
  • Kaposi's sarcoma
  • Plasma viraemia

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

HHV-8 DNA replication correlates with the clinical status in AIDS-related Kaposi's sarcoma. / Broccolo, Francesco; Tassan Din, Chiara; Viganò, Maria Grazia; Rutigliano, Teresa; Esposito, Susanna; Lusso, P.; Tambussi, Giuseppe; Malnati, Mauro.

In: Journal of Clinical Virology, Vol. 78, 01.05.2016, p. 47-52.

Research output: Contribution to journalArticle

Broccolo, Francesco ; Tassan Din, Chiara ; Viganò, Maria Grazia ; Rutigliano, Teresa ; Esposito, Susanna ; Lusso, P. ; Tambussi, Giuseppe ; Malnati, Mauro. / HHV-8 DNA replication correlates with the clinical status in AIDS-related Kaposi's sarcoma. In: Journal of Clinical Virology. 2016 ; Vol. 78. pp. 47-52.
@article{59807b5cb1644074b45d47106152816c,
title = "HHV-8 DNA replication correlates with the clinical status in AIDS-related Kaposi's sarcoma",
abstract = "Background: The value of plasma levels of human herpesvirus 8 (HHV-8) DNA as a marker of clinical status in acquired immunodeficiency syndrome-related Kaposi's sarcoma (AIDS-KS) remains to be elucidated. Objectives: To investigate the relationship between the plasma HHV-8 DNA viral load and the clinical status of AIDS-KS. Study Design: A total of 378 blood samples were obtained from 62 patients with AIDS-KS followed longitudinally. All patients received antiretroviral therapy (ART) or anti-neoplastic therapy. The patients were divided into four groups according to their clinical status: onset disease (OD), progressive disease (PD), stable or partial remission (S/PR) and complete remission (CR). Results: Plasma HHV-8 DNAaemia was detected in all samples obtained from patients with OD or PD (100{\%}); in contrast, HHV-8 DNAaemia was found only in a minority of patients with CR (8{\%}) and was invariably undetectable in patients with stable CR. HHV-8 DNA detection in plasma was strongly associated with an unfavourable outcome (odds ratio = 231.9; p <0.0001). Conversely, neither the HIV-1 viral load nor peripheral CD4+ T-cell counts were associated with the KS clinical status, though both parameters did affect HHV-8 DNAaemia levels (p <0.0001). Multivariate analysis confirmed that HHV-8 DNAaemia was strongly and independently correlated with both clinical status (p <0.05) and HIV-1 plasma viraemia (p = 0.027). Conclusions: The strong association of plasma HHV-8 DNAaemia with onset or progressive disease is compatible with an active role of replicating virus in clinically active AIDS-KS. An accurate evaluation of the plasma HHV-8 load might be useful for monitoring AIDS-KS under antiretroviral or antineoplastic therapy.",
keywords = "Disease progression, Human herpesvirus-8, Kaposi's sarcoma, Plasma viraemia",
author = "Francesco Broccolo and {Tassan Din}, Chiara and Vigan{\`o}, {Maria Grazia} and Teresa Rutigliano and Susanna Esposito and P. Lusso and Giuseppe Tambussi and Mauro Malnati",
year = "2016",
month = "5",
day = "1",
doi = "10.1016/j.jcv.2016.02.019",
language = "English",
volume = "78",
pages = "47--52",
journal = "Journal of Clinical Virology",
issn = "1386-6532",
publisher = "Elsevier",

}

TY - JOUR

T1 - HHV-8 DNA replication correlates with the clinical status in AIDS-related Kaposi's sarcoma

AU - Broccolo, Francesco

AU - Tassan Din, Chiara

AU - Viganò, Maria Grazia

AU - Rutigliano, Teresa

AU - Esposito, Susanna

AU - Lusso, P.

AU - Tambussi, Giuseppe

AU - Malnati, Mauro

PY - 2016/5/1

Y1 - 2016/5/1

N2 - Background: The value of plasma levels of human herpesvirus 8 (HHV-8) DNA as a marker of clinical status in acquired immunodeficiency syndrome-related Kaposi's sarcoma (AIDS-KS) remains to be elucidated. Objectives: To investigate the relationship between the plasma HHV-8 DNA viral load and the clinical status of AIDS-KS. Study Design: A total of 378 blood samples were obtained from 62 patients with AIDS-KS followed longitudinally. All patients received antiretroviral therapy (ART) or anti-neoplastic therapy. The patients were divided into four groups according to their clinical status: onset disease (OD), progressive disease (PD), stable or partial remission (S/PR) and complete remission (CR). Results: Plasma HHV-8 DNAaemia was detected in all samples obtained from patients with OD or PD (100%); in contrast, HHV-8 DNAaemia was found only in a minority of patients with CR (8%) and was invariably undetectable in patients with stable CR. HHV-8 DNA detection in plasma was strongly associated with an unfavourable outcome (odds ratio = 231.9; p <0.0001). Conversely, neither the HIV-1 viral load nor peripheral CD4+ T-cell counts were associated with the KS clinical status, though both parameters did affect HHV-8 DNAaemia levels (p <0.0001). Multivariate analysis confirmed that HHV-8 DNAaemia was strongly and independently correlated with both clinical status (p <0.05) and HIV-1 plasma viraemia (p = 0.027). Conclusions: The strong association of plasma HHV-8 DNAaemia with onset or progressive disease is compatible with an active role of replicating virus in clinically active AIDS-KS. An accurate evaluation of the plasma HHV-8 load might be useful for monitoring AIDS-KS under antiretroviral or antineoplastic therapy.

AB - Background: The value of plasma levels of human herpesvirus 8 (HHV-8) DNA as a marker of clinical status in acquired immunodeficiency syndrome-related Kaposi's sarcoma (AIDS-KS) remains to be elucidated. Objectives: To investigate the relationship between the plasma HHV-8 DNA viral load and the clinical status of AIDS-KS. Study Design: A total of 378 blood samples were obtained from 62 patients with AIDS-KS followed longitudinally. All patients received antiretroviral therapy (ART) or anti-neoplastic therapy. The patients were divided into four groups according to their clinical status: onset disease (OD), progressive disease (PD), stable or partial remission (S/PR) and complete remission (CR). Results: Plasma HHV-8 DNAaemia was detected in all samples obtained from patients with OD or PD (100%); in contrast, HHV-8 DNAaemia was found only in a minority of patients with CR (8%) and was invariably undetectable in patients with stable CR. HHV-8 DNA detection in plasma was strongly associated with an unfavourable outcome (odds ratio = 231.9; p <0.0001). Conversely, neither the HIV-1 viral load nor peripheral CD4+ T-cell counts were associated with the KS clinical status, though both parameters did affect HHV-8 DNAaemia levels (p <0.0001). Multivariate analysis confirmed that HHV-8 DNAaemia was strongly and independently correlated with both clinical status (p <0.05) and HIV-1 plasma viraemia (p = 0.027). Conclusions: The strong association of plasma HHV-8 DNAaemia with onset or progressive disease is compatible with an active role of replicating virus in clinically active AIDS-KS. An accurate evaluation of the plasma HHV-8 load might be useful for monitoring AIDS-KS under antiretroviral or antineoplastic therapy.

KW - Disease progression

KW - Human herpesvirus-8

KW - Kaposi's sarcoma

KW - Plasma viraemia

UR - http://www.scopus.com/inward/record.url?scp=84960398792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960398792&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2016.02.019

DO - 10.1016/j.jcv.2016.02.019

M3 - Article

VL - 78

SP - 47

EP - 52

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

ER -