Abstract
The HIC1 gene is a transcriptional regulator commonly methylated in a variety of human cancer. Thirty-three invasive ductal carcinomas of the breast and 21 matched normal breast tissues were analysed for HIC1 promoter methylation, and allelic loss of a 700 kb region spanning the gene locus. At least one genetic or epigenetic abnormality was found in 27 of the carcinomas tested (82%). Promoter methylation was demonstrated in 21 carcinomas (64%), and nine normal tissues (43%), whereas 18 malignant tumors (54%) showed allelic loss. Concomitant loss of heterozigosity and promoter hypermethylation in the region spanning HIC1 was detected in eight carcinomas (24%) suggesting that in this subset of tumors both copies of the gene are functionally lost. These observations support a role for the HIC1 gene in the pathogenesis of breast ductal carcinomas.
Original language | English |
---|---|
Pages (from-to) | 75-81 |
Number of pages | 7 |
Journal | Cancer Letters |
Volume | 222 |
Issue number | 1 |
DOIs | |
Publication status | Published - May 10 2005 |
Keywords
- Breast cancer
- Chromosome 17p13
- DNA methylation
- HIC1
- LOH
- Tumor suppressor genes
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology