HIC1 promoter methylation and 17p13.3 allelic loss in invasive ductal carcinoma of the breast

Paola Parrella, Marina Scintu, Maria Prencipe, Maria Luana Poeta, Antonietta Pia Gallo, Carla Rabitti, Monica Rinaldi, Stefania Tommasi, Angelo Paradiso, Francesco Schittulli, Vanna Maria Valori, Salvatore Toma, Vittorio Altomare, Vito Michele Fazio

Research output: Contribution to journalArticle

Abstract

The HIC1 gene is a transcriptional regulator commonly methylated in a variety of human cancer. Thirty-three invasive ductal carcinomas of the breast and 21 matched normal breast tissues were analysed for HIC1 promoter methylation, and allelic loss of a 700 kb region spanning the gene locus. At least one genetic or epigenetic abnormality was found in 27 of the carcinomas tested (82%). Promoter methylation was demonstrated in 21 carcinomas (64%), and nine normal tissues (43%), whereas 18 malignant tumors (54%) showed allelic loss. Concomitant loss of heterozigosity and promoter hypermethylation in the region spanning HIC1 was detected in eight carcinomas (24%) suggesting that in this subset of tumors both copies of the gene are functionally lost. These observations support a role for the HIC1 gene in the pathogenesis of breast ductal carcinomas.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalCancer Letters
Volume222
Issue number1
DOIs
Publication statusPublished - May 10 2005

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Keywords

  • Breast cancer
  • Chromosome 17p13
  • DNA methylation
  • HIC1
  • LOH
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Parrella, P., Scintu, M., Prencipe, M., Poeta, M. L., Gallo, A. P., Rabitti, C., Rinaldi, M., Tommasi, S., Paradiso, A., Schittulli, F., Valori, V. M., Toma, S., Altomare, V., & Fazio, V. M. (2005). HIC1 promoter methylation and 17p13.3 allelic loss in invasive ductal carcinoma of the breast. Cancer Letters, 222(1), 75-81. https://doi.org/10.1016/j.canlet.2004.08.026