TY - JOUR
T1 - High aminopeptidase N/CD13 levels characterize human amniotic mesenchymal stem cells and drive their increased adipogenic potential in obese women
AU - Iaffaldano, Laura
AU - Nardelli, Carmela
AU - Raia, Maddalena
AU - Mariotti, Elisabetta
AU - Ferrigno, Maddalena
AU - Quaglia, Filomena
AU - Labruna, Giuseppe
AU - Capobianco, Valentina
AU - Capone, Angela
AU - Maruotti, Giuseppe Maria
AU - Pastore, Lucio
AU - Di Noto, Rosa
AU - Martinelli, Pasquale
AU - Sacchetti, Lucia
AU - Del Vecchio, Luigi
PY - 2013/8/15
Y1 - 2013/8/15
N2 - Maternal obesity is associated to increased fetal risk of obesity and other metabolic diseases. Human amniotic mesenchymal stem cells (hA-MSCs) have not been characterized in obese women. The aim of this study was to isolate and compare hA-MSC immunophenotypes from obese (Ob-) and normal weight control (Co-) women, to identify alterations possibly predisposing the fetus to obesity. We enrolled 16 Ob- and 7 Co-women at delivery (mean/SEM prepregnancy body mass index: 40.3/1.8 and 22.4/1.0 kg/m2, respectively), and 32 not pregnant women. hA-MSCs were phenotyped by flow cytometry; several maternal and newborn clinical and biochemical parameters were also measured. The expression of membrane antigen CD13 was higher on Ob-hA-MSCs than on Co-hA-MSCs (P=0.005). Also, serum levels of CD13 at delivery were higher in Ob- versus Co-pregnant women and correlated with CD13 antigen expression on Ob-hA-MSCs (r 2=0.84, P
AB - Maternal obesity is associated to increased fetal risk of obesity and other metabolic diseases. Human amniotic mesenchymal stem cells (hA-MSCs) have not been characterized in obese women. The aim of this study was to isolate and compare hA-MSC immunophenotypes from obese (Ob-) and normal weight control (Co-) women, to identify alterations possibly predisposing the fetus to obesity. We enrolled 16 Ob- and 7 Co-women at delivery (mean/SEM prepregnancy body mass index: 40.3/1.8 and 22.4/1.0 kg/m2, respectively), and 32 not pregnant women. hA-MSCs were phenotyped by flow cytometry; several maternal and newborn clinical and biochemical parameters were also measured. The expression of membrane antigen CD13 was higher on Ob-hA-MSCs than on Co-hA-MSCs (P=0.005). Also, serum levels of CD13 at delivery were higher in Ob- versus Co-pregnant women and correlated with CD13 antigen expression on Ob-hA-MSCs (r 2=0.84, P
UR - http://www.scopus.com/inward/record.url?scp=84880873761&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880873761&partnerID=8YFLogxK
U2 - 10.1089/scd.2012.0499
DO - 10.1089/scd.2012.0499
M3 - Article
C2 - 23488598
AN - SCOPUS:84880873761
VL - 22
SP - 2287
EP - 2297
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 16
ER -