TY - JOUR
T1 - High concordance of KRAS status between primary colorectal tumors and related metastatic sites
T2 - Implications for clinical practice
AU - Santini, Daniele
AU - Loupakis, Fotios
AU - Vincenzi, Bruno
AU - Floriani, Irene
AU - Stasi, Irene
AU - Canestrari, Emanuele
AU - Rulli, Eliana
AU - Maltese, Paolo Enrico
AU - Andreoni, Francesca
AU - Masi, Gianluca
AU - Graziano, Francesco
AU - Baldi, Giacomo Giulio
AU - Salvatore, Lisa
AU - Russo, Antonio
AU - Perrone, Giuseppe
AU - Tommasino, Maria Rosa
AU - Magnani, Mauro
AU - Falcone, Alfredo
AU - Tonini, Giuseppe
AU - Ruzzo, Annamaria
PY - 2008/12
Y1 - 2008/12
N2 - Purpose. Several studies have suggested that KRAS somatic mutations may predict resistance to cetuximab-and panitumumab-based treatments in metastatic colorectal cancer (CRC) patients. Nevertheless, most experiences were conducted on samples from primaries. The aim of this study was to evaluate the grade of concordance in terms of KRAS status between primaries and related metastases. Patients and Methods. We analyzed KRAS codon 12 and 13 mutations from formalin-fixed sections of 107 CRC primaries and related metastases. Eight pairs were excluded from the analysis because of the low amount of tumor tissue in the available samples. The main characteristics were: 50 men, 49 women; median age at diagnosis, 71 years (range, 41-84). The metastatic sites analyzed were the liver in 80 patients (80.8%), lung in seven patients (7.1%), and other sites in 12 patients (12.1%). Results. A KRAS mutation was found in 38 (38.4%) primary tumors and in 36 (36.4%) related metastases. The rate of concordance was 96.0% (95% confidence interval, 90.0%-98.9%). Discordance was observed in only four (4%) patients. Conclusions. Our results indicate that the detection of KRAS mutations in either primary or metastatic tumors from patients with CRC is concordant and this assessment could be used to predict response to targeted therapies such as cetuximab and panitumumab.
AB - Purpose. Several studies have suggested that KRAS somatic mutations may predict resistance to cetuximab-and panitumumab-based treatments in metastatic colorectal cancer (CRC) patients. Nevertheless, most experiences were conducted on samples from primaries. The aim of this study was to evaluate the grade of concordance in terms of KRAS status between primaries and related metastases. Patients and Methods. We analyzed KRAS codon 12 and 13 mutations from formalin-fixed sections of 107 CRC primaries and related metastases. Eight pairs were excluded from the analysis because of the low amount of tumor tissue in the available samples. The main characteristics were: 50 men, 49 women; median age at diagnosis, 71 years (range, 41-84). The metastatic sites analyzed were the liver in 80 patients (80.8%), lung in seven patients (7.1%), and other sites in 12 patients (12.1%). Results. A KRAS mutation was found in 38 (38.4%) primary tumors and in 36 (36.4%) related metastases. The rate of concordance was 96.0% (95% confidence interval, 90.0%-98.9%). Discordance was observed in only four (4%) patients. Conclusions. Our results indicate that the detection of KRAS mutations in either primary or metastatic tumors from patients with CRC is concordant and this assessment could be used to predict response to targeted therapies such as cetuximab and panitumumab.
KW - Colorectal cancer
KW - Concordance
KW - Kras mutations
KW - Metastatic sites
KW - Primary tumors
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U2 - 10.1634/theoncologist.2008-0181
DO - 10.1634/theoncologist.2008-0181
M3 - Article
C2 - 19056857
AN - SCOPUS:58749102224
VL - 13
SP - 1270
EP - 1275
JO - Oncologist
JF - Oncologist
SN - 1083-7159
IS - 12
ER -