TY - JOUR
T1 - High-Dimensional Single-Cell Quantitative Profiling of Skeletal Muscle Cell Population Dynamics during Regeneration
AU - Petrilli, Lucia Lisa
AU - Spada, Filomena
AU - Palma, Alessandro
AU - Reggio, Alessio
AU - Rosina, Marco
AU - Gargioli, Cesare
AU - Castagnoli, Luisa
AU - Fuoco, Claudia
AU - Cesareni, Gianni
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2020/7/18
Y1 - 2020/7/18
N2 - The interstitial space surrounding the skeletal muscle fibers is populated by a variety of mononuclear cell types. Upon acute or chronic insult, these cell populations become activated and initiate finely-orchestrated crosstalk that promotes myofiber repair and regeneration. Mass cytometry is a powerful and highly multiplexed technique for profiling single-cells. Herein, it was used to dissect the dynamics of cell populations in the skeletal muscle in physiological and pathological conditions. Here, we characterized an antibody panel that could be used to identify most of the cell populations in the muscle interstitial space. By exploiting the mass cytometry resolution, we provided a comprehensive picture of the dynamics of the major cell populations that sensed and responded to acute damage in wild type mice and in a mouse model of Duchenne muscular dystrophy. In addition, we revealed the intrinsic heterogeneity of many of these cell populations.
AB - The interstitial space surrounding the skeletal muscle fibers is populated by a variety of mononuclear cell types. Upon acute or chronic insult, these cell populations become activated and initiate finely-orchestrated crosstalk that promotes myofiber repair and regeneration. Mass cytometry is a powerful and highly multiplexed technique for profiling single-cells. Herein, it was used to dissect the dynamics of cell populations in the skeletal muscle in physiological and pathological conditions. Here, we characterized an antibody panel that could be used to identify most of the cell populations in the muscle interstitial space. By exploiting the mass cytometry resolution, we provided a comprehensive picture of the dynamics of the major cell populations that sensed and responded to acute damage in wild type mice and in a mouse model of Duchenne muscular dystrophy. In addition, we revealed the intrinsic heterogeneity of many of these cell populations.
KW - fibro/adipogenic progenitors
KW - mass cytometry
KW - muscle populations
KW - myogenic progenitors
KW - single-cell
KW - skeletal muscle homeostasis
KW - skeletal muscle regeneration
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U2 - 10.3390/cells9071723
DO - 10.3390/cells9071723
M3 - Article
C2 - 32708412
AN - SCOPUS:85088679444
VL - 9
JO - Cells
JF - Cells
SN - 2073-4409
IS - 7
ER -