High-efficiency antibody discovery achieved with multiplexed microscopy

Shelley Mettler Izquierdo, Stephanie Varela, Minha Park, Ellen J. Collarini, Daniel Lu, Shreya Pramanick, Joseph Rucker, Lucia Lopalco, Rob Etches, William Harriman

Research output: Contribution to journalArticle

Abstract

The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully leveraged by accessing primary B-cell populations from evolutionarily divergent species such as chickens.

Original languageEnglish
Pages (from-to)341-352
Number of pages12
JournalMicroscopy (Oxford, England)
Volume65
Issue number4
DOIs
Publication statusPublished - Aug 1 2016

Keywords

  • Antibody discovery
  • Chicken monoclonal antibody
  • DR4 DR5
  • GPCR
  • Ion channel
  • Single b cell cloning

ASJC Scopus subject areas

  • Structural Biology
  • Medicine(all)
  • Instrumentation
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Izquierdo, S. M., Varela, S., Park, M., Collarini, E. J., Lu, D., Pramanick, S., Rucker, J., Lopalco, L., Etches, R., & Harriman, W. (2016). High-efficiency antibody discovery achieved with multiplexed microscopy. Microscopy (Oxford, England), 65(4), 341-352. https://doi.org/10.1093/jmicro/dfw014