Abstract
Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to "knockdown" gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporationbased approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
Original language | English |
---|---|
Pages (from-to) | 27-31 |
Number of pages | 5 |
Journal | Genesis |
Volume | 32 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2002 |
Keywords
- Dominant-negative mutation
- Electroporation
- Fgf
- Fgf receptor 1
- Fin
- Regeneration
- Zebrafish
ASJC Scopus subject areas
- Genetics