Abstract
Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to "knockdown" gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporationbased approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 27-31 |
| Number of pages | 5 |
| Journal | Genesis |
| Volume | 32 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Dominant-negative mutation
- Electroporation
- Fgf
- Fgf receptor 1
- Fin
- Regeneration
- Zebrafish
ASJC Scopus subject areas
- Genetics
Cite this
High-efficiency gene transfer into adult fish : A new tool to study fin regeneration. / Tawk, Marcel; Tuil, David; Torrente, Yvan; Vriz, Sophie; Paulin, Denise.
In: Genesis, Vol. 32, No. 1, 2002, p. 27-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-efficiency gene transfer into adult fish
T2 - A new tool to study fin regeneration
AU - Tawk, Marcel
AU - Tuil, David
AU - Torrente, Yvan
AU - Vriz, Sophie
AU - Paulin, Denise
PY - 2002
Y1 - 2002
N2 - Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to "knockdown" gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporationbased approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
AB - Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to "knockdown" gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporationbased approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
KW - Dominant-negative mutation
KW - Electroporation
KW - Fgf
KW - Fgf receptor 1
KW - Fin
KW - Regeneration
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=0036161342&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036161342&partnerID=8YFLogxK
U2 - 10.1002/gene.10025
DO - 10.1002/gene.10025
M3 - Article
C2 - 11835671
AN - SCOPUS:0036161342
VL - 32
SP - 27
EP - 31
JO - Genesis
JF - Genesis
SN - 1526-954X
IS - 1
ER -