High-efficiency gene transfer into adult fish: A new tool to study fin regeneration

Marcel Tawk, David Tuil, Yvan Torrente, Sophie Vriz, Denise Paulin

Research output: Contribution to journalArticlepeer-review

Abstract

Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to "knockdown" gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporationbased approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalGenesis
Volume32
Issue number1
DOIs
Publication statusPublished - 2002

Keywords

  • Dominant-negative mutation
  • Electroporation
  • Fgf
  • Fgf receptor 1
  • Fin
  • Regeneration
  • Zebrafish

ASJC Scopus subject areas

  • Genetics

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