High efficiency preparation of L-[S-methyl-11C]methionine by on-column [11C]methylation on C18 Sep-Pak

Claudio Pascali, Anna Bogni, Ren Iwata, Donatella Decise, Flavio Crippa, Emilio Bombardieri

Research output: Contribution to journalArticlepeer-review


A novel approach to the synthesis of L-[S-methyl-11C]methionine, (11C]MET) is described which involves a commercial C18 Sep-Pak Plus as a solid-phase support material for the [11C]methylation step. The present method, which uses [11C]CH3I produced by the classical LiAlH4/HI route, supplies [11C]MET ready for injection within 11 min from the end of bombardment (EOB) with a radiochemical yield, decay corrected at EOB, of 78% and a radiochemical purity at the end of synthesis (EOS) higher than 99%. The required setup is extremely simple and easy to automate and can be reset for a further synthesis within few minutes. Moreover, due to its versatility, the method can be utilized for the production of other radiopharmaceuticals requiring a simple [11C]methylation.

Original languageEnglish
Pages (from-to)715-724
Number of pages10
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Issue number8
Publication statusPublished - 1999


  • [C]methionine
  • On-column reaction
  • PET

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology


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