High-frequency and adaptive-like dynamics of human CD1 self-reactive T cells

Claudia De Lalla, Marco Lepore, Francesco M. Piccolo, Anna Rinaldi, Andrea Scelfo, Claudio Garavaglia, Lucia Mori, Gennaro De Libero, Paolo Dellabona, Giulia Casorati

Research output: Contribution to journalArticle

Abstract

CD1 molecules present lipid antigens to T cells. An intriguing subset of human T cells recognize CD1-expressing cells without deliberately added lipids. Frequency, subset distribution, clonal composition, naïve-to-memory dynamic transition of these CD1 self-reactive T cells remain largely unknown. By screening libraries of T-cell clones, generated from CD4 + or CD4 -CD8 - double negative (DN) T cells sorted from the same donors, and by limiting dilution analysis, we find that the frequency of CD1 self-reactive T cells is unexpectedly high in both T-cell subsets, in the range of 1/10-1/300 circulating T cells. These T cells predominantly recognize CD1a and CD1c and express diverse TCRs. Frequency comparisons of T-cell clones from sorted naïve and memory compartments of umbilical cord and adult blood show that CD1 self-reactive T cells are naïve at birth and undergo an age-dependent increase in the memory compartment, suggesting a naïve/memory adaptive-like population dynamics. CD1 self-reactive clones exhibit mostly Th1 and Th0 functional activities, depending on the subset and on the CD1 isotype restriction. These findings unveil the unanticipated relevance of self-lipid T-cell response in humans and clarify the basic parameters of the lipid-specific T-cell physiology.

Original languageEnglish
Pages (from-to)602-610
Number of pages9
JournalEuropean Journal of Immunology
Volume41
Issue number3
DOIs
Publication statusPublished - Mar 2011

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T-Lymphocytes
Lipids
Clone Cells
T-Lymphocyte Subsets
Cell Physiological Phenomena
Population Dynamics
Fetal Blood
Parturition
Antigens

Keywords

  • CD1 molecules
  • Cellular immunology
  • Immune response
  • T cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

High-frequency and adaptive-like dynamics of human CD1 self-reactive T cells. / De Lalla, Claudia; Lepore, Marco; Piccolo, Francesco M.; Rinaldi, Anna; Scelfo, Andrea; Garavaglia, Claudio; Mori, Lucia; De Libero, Gennaro; Dellabona, Paolo; Casorati, Giulia.

In: European Journal of Immunology, Vol. 41, No. 3, 03.2011, p. 602-610.

Research output: Contribution to journalArticle

De Lalla, Claudia ; Lepore, Marco ; Piccolo, Francesco M. ; Rinaldi, Anna ; Scelfo, Andrea ; Garavaglia, Claudio ; Mori, Lucia ; De Libero, Gennaro ; Dellabona, Paolo ; Casorati, Giulia. / High-frequency and adaptive-like dynamics of human CD1 self-reactive T cells. In: European Journal of Immunology. 2011 ; Vol. 41, No. 3. pp. 602-610.
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