High frequency of Merkel cell polyomavirus DNA in the urine of kidney transplant recipients and healthy controls

Lucia Signorini, Mirco Belingheri, Federico Ambrogi, Elisabetta Pagani, Sandro Binda, Rosalia Ticozzi, Mariano Ferraresso, Luciana Ghio, Bruno Giacon, Pasquale Ferrante, Serena Delbue

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Abstract

Background: Polyomavirus (PyV) infection is common, ranging from 60% to 100% depending on the virus. The urinary excretion rates of JC virus (JCV) and BK virus (BKV) have been extensively studied, but less is known about the more recently discovered PyVs. Objectives: To investigate the urinary excretion of Merkel cell PyV (MCPyV), which is associated with Merkel cell carcinoma (MCC), in kidney transplant recipients and healthy subjects, as well as those of lymphotropic polyomavirus (LPV), which was isolated from the B-cells of African green monkeys but has also been found in human blood, JCV and BKV. Study design: Urine samples were collected from 62 adult (ATP) and 46 pediatric (PTP) kidney transplant recipients and from 67 adult (AHC) and 40 pediatric (PHC) healthy controls. DNA was isolated and analyzed using real-time PCR (Q-PCR) to determine the viral loads of MCPyV, LPV, JCV and BKV. Results: MCPyV DNA was more frequently detected (p

Original languageEnglish
Pages (from-to)565-570
Number of pages6
JournalJournal of Clinical Virology
Volume61
Issue number4
DOIs
Publication statusPublished - 2014

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Keywords

  • Kidney transplant recipients
  • Merkel cell polyomavirus
  • Polyomaviruses
  • Urinary excretion

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Signorini, L., Belingheri, M., Ambrogi, F., Pagani, E., Binda, S., Ticozzi, R., Ferraresso, M., Ghio, L., Giacon, B., Ferrante, P., & Delbue, S. (2014). High frequency of Merkel cell polyomavirus DNA in the urine of kidney transplant recipients and healthy controls. Journal of Clinical Virology, 61(4), 565-570. https://doi.org/10.1016/j.jcv.2014.10.012