TY - JOUR
T1 - High-Frequency Ultrasound for the Study of Early Mouse Embryonic Cardiovascular System
AU - Greco, Adelaide
AU - Coda, Anna Rita Daniela
AU - Albanese, Sandra
AU - Ragucci, Monica
AU - Liuzzi, Raffaele
AU - Auletta, Luigi
AU - Gargiulo, Sara
AU - Lamagna, Francesco
AU - Salvatore, Marco
AU - Mancini, Marcello
PY - 2015/12/1
Y1 - 2015/12/1
N2 - An accurate diagnosis of congenital heart defects during fetal development is critical for interventional planning. Mice can be used to generate animal models with heart defects, and high-frequency ultrasound (HFUS) imaging enables in utero imaging of live mouse embryos. A wide range of physiological measurements is possible using Doppler-HFUS imaging; limitations of any single measurement warrant a multiparameter approach to characterize cardiovascular function. Doppler-HFUS was used to explore the embryonic (heart, aorta) and extraembryonic (umbilical blood flow) circulatory systems to create a database in normal mouse embryos between 9.5 and 16.5 days of gestation. Multivariate analyses were performed to explore correlations between gestational age and embryo echocardiographic parameters. Heart rate and peak velocity in the aorta were positively correlated with gestational time, whereas cardiac cycle length, isovolumetric relaxation time, myocardial performance index, and arterial deceleration time of the umbilical cord were negatively correlated with it. Doppler-HFUS facilitated detailed characterization of the embryonic mouse circulation and represents a useful tool for investigation of the early mouse embryonic cardiovascular system.
AB - An accurate diagnosis of congenital heart defects during fetal development is critical for interventional planning. Mice can be used to generate animal models with heart defects, and high-frequency ultrasound (HFUS) imaging enables in utero imaging of live mouse embryos. A wide range of physiological measurements is possible using Doppler-HFUS imaging; limitations of any single measurement warrant a multiparameter approach to characterize cardiovascular function. Doppler-HFUS was used to explore the embryonic (heart, aorta) and extraembryonic (umbilical blood flow) circulatory systems to create a database in normal mouse embryos between 9.5 and 16.5 days of gestation. Multivariate analyses were performed to explore correlations between gestational age and embryo echocardiographic parameters. Heart rate and peak velocity in the aorta were positively correlated with gestational time, whereas cardiac cycle length, isovolumetric relaxation time, myocardial performance index, and arterial deceleration time of the umbilical cord were negatively correlated with it. Doppler-HFUS facilitated detailed characterization of the embryonic mouse circulation and represents a useful tool for investigation of the early mouse embryonic cardiovascular system.
KW - cardiovascular
KW - mice
KW - ultrasound
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U2 - 10.1177/1933719115594017
DO - 10.1177/1933719115594017
M3 - Article
AN - SCOPUS:84947080785
VL - 22
SP - 1649
EP - 1655
JO - Reproductive Sciences
JF - Reproductive Sciences
SN - 1933-7191
IS - 12
ER -