High-fructose diet decreases catalase mRNA levels in rat tissues

A. Cavarape, F. Feletto, F. Mercuri, L. Quagliaro, G. Damante, A. Ceriello

Research output: Contribution to journalArticlepeer-review


Insulin resistance and hyperinsulinemia have recently been identified as independent determinants of several risk factors for cardiovascular disease. The generation of reactive oxygen species (ROS) may play an important role as a final common mediator by which glucose and insulin resistance might contribute to development of cardiovascular disease and hypertension. The aim of the present study was to evaluate changes on mRNA expression of antioxidant enzymes [catalase, Cu-Zn superoxide dismutase (Cu-ZnSOD), MnSOD], blood pressure and metabolic parameters in insulin resistance that follow feeding normotensive Wistar rats a high-fructose-enriched diet. In our investigation 26 normal male Wistar rats were fed a high-fructose diet for 2 weeks (no.=14) or normal chow to serve as a control group (no.=12). In vivo insulin resistance was verified in a subgroup of control and fructose-fed rats by the euglycemic hyperinsulinemic clamp technique at 2 different insulin infusion rates, 29 (submaximal stimulation) and 290 (maximal stimulation) pmol/kg/min respectively. The glucose infusion rate (GIR) was not significantly different in the two groups during the submaximal infusion of insulin (1.4±0.8 mmol/kg/min in fructose-fed rats vs 1.6±0.7 mmol/kg/min in control rats, NS) while in fructose-fed rats it was significantly lower (-29.8%) than in control rats during maximal infusion of insulin (2.6±0.3 mmol/kg/min vs 3.7±0.3 mmol/kg/min, p

Original languageEnglish
Pages (from-to)838-845
Number of pages8
JournalJournal of Endocrinological Investigation
Issue number11
Publication statusPublished - 2001


  • Antioxidant enzymes
  • Catalase
  • High-fructose diet
  • Insulin resistance
  • Oxidative stress

ASJC Scopus subject areas

  • Endocrinology


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