High genetic instability of heterochromatin after transposition of the LINE-like I factor in Drosophila melanogaster

Patrizio Dimitri, Bruno Arcà, Libera Berghella, Elisabetta Mei

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

In the present work, we have asked whether a group of 13 essential genes mapping to the heterochromatin of Drosophila melanogaster chromosome 2 are mutable following transposition of the I factor during I-R hybrid dysgenesis. We found that the frequency of lethal events mapping to chromosome 2 heterochromatin is surprisingly high, despite the low density of genetic functions identified in this region compared with euchromatin. Cytogenetic and molecular analyses indicated that the recovered mutations correspond either to insertions or to rearrangements. Moreover, chromosomes bearing specific heterochromatic lethal mutations were generated by recombination in the heterochromatin. Together, these data indicate that I factors transpose with high frequency into pericentric regions of chromosome 2 and may play a role in the evolution of constitutive heterochromatin.

Original languageEnglish
Pages (from-to)8052-8057
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number15
DOIs
Publication statusPublished - Jul 22 1997

Fingerprint

Heterochromatin
Drosophila melanogaster
Chromosomes, Human, Pair 2
Euchromatin
Mutation
Chromosome Mapping
Cytogenetic Analysis
Essential Genes
Genetic Recombination
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

High genetic instability of heterochromatin after transposition of the LINE-like I factor in Drosophila melanogaster. / Dimitri, Patrizio; Arcà, Bruno; Berghella, Libera; Mei, Elisabetta.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 15, 22.07.1997, p. 8052-8057.

Research output: Contribution to journalArticle

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