High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay

Giuseppe Criseo, Cosimo Racco, Orazio Romeo

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Aflatoxigenic Aspergillus flavus isolates always show, by using a multiplex PCR-system, four DNA fragments specific for aflR, nor-1, ver-1, and omt-A genes. Non-aflatoxigenic A. flavus strains give variable DNA banding pattern lacking one, two, three or four of these genes. Recently, it has been found and reported that some aflatoxin non-producing A. flavus strains show a complete set of genes. Because less is known about the incidence of structural genes aflR, nor-1, ver-1 and omt-A in aflatoxin non-producing strains of A. flavus, we decided to study the frequencies of the aflatoxin structural genes in non-aflatoxigenic A. flavus strains isolated from food and feed commodities. The results can be summarized as following: 36.5% of the examined non-aflatoxigenic A. flavus strains showed DNA fragments that correspond to the complete set of genes (quadruplet pattern) as found in aflatoxigenic A. flavus. Forty three strains (32%) showed three DNA banding patterns grouped in four profiles where nor-1, ver-1 and omt-A was the most frequent profile. Twenty five (18.7%) of non-aflatoxigenic A. flavus strains yielded two DNA banding pattern whereas sixteen (12%) of the strains showed one DNA banding pattern. In one strain, isolated from poultry feed, no DNA bands were found. The nor-1 gene was the most representative between the four aflatoxin structural assayed genes. Lower incidence was found for aflR gene. Our data show a high level of genetic variability among non-aflatoxigenic A. flavus isolates that require greater attention in order to design molecular experiment to distinguish true aflatoxigenic from non-aflatoxigenic A. flavus strains.

Original languageEnglish
Pages (from-to)341-343
Number of pages3
JournalInternational Journal of Food Microbiology
Volume125
Issue number3
DOIs
Publication statusPublished - Jul 31 2008

Fingerprint

Aspergillus flavus
Polymerase Chain Reaction
genetic variation
assays
Aflatoxins
DNA
aflatoxins
Genes
structural genes
genes
vif Genes
incidence
Multiplex Polymerase Chain Reaction
Incidence
Poultry
products and commodities
poultry
Food

Keywords

  • Aflatoxin
  • Aflatoxin non-producing A. flavus strains
  • Aspergillus flavus
  • nor-1, omt-A, ver-1 and aflR genes

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay. / Criseo, Giuseppe; Racco, Cosimo; Romeo, Orazio.

In: International Journal of Food Microbiology, Vol. 125, No. 3, 31.07.2008, p. 341-343.

Research output: Contribution to journalArticle

Criseo, Giuseppe ; Racco, Cosimo ; Romeo, Orazio. / High genetic variability in non-aflatoxigenic A. flavus strains by using Quadruplex PCR-based assay. In: International Journal of Food Microbiology. 2008 ; Vol. 125, No. 3. pp. 341-343.
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AB - Aflatoxigenic Aspergillus flavus isolates always show, by using a multiplex PCR-system, four DNA fragments specific for aflR, nor-1, ver-1, and omt-A genes. Non-aflatoxigenic A. flavus strains give variable DNA banding pattern lacking one, two, three or four of these genes. Recently, it has been found and reported that some aflatoxin non-producing A. flavus strains show a complete set of genes. Because less is known about the incidence of structural genes aflR, nor-1, ver-1 and omt-A in aflatoxin non-producing strains of A. flavus, we decided to study the frequencies of the aflatoxin structural genes in non-aflatoxigenic A. flavus strains isolated from food and feed commodities. The results can be summarized as following: 36.5% of the examined non-aflatoxigenic A. flavus strains showed DNA fragments that correspond to the complete set of genes (quadruplet pattern) as found in aflatoxigenic A. flavus. Forty three strains (32%) showed three DNA banding patterns grouped in four profiles where nor-1, ver-1 and omt-A was the most frequent profile. Twenty five (18.7%) of non-aflatoxigenic A. flavus strains yielded two DNA banding pattern whereas sixteen (12%) of the strains showed one DNA banding pattern. In one strain, isolated from poultry feed, no DNA bands were found. The nor-1 gene was the most representative between the four aflatoxin structural assayed genes. Lower incidence was found for aflR gene. Our data show a high level of genetic variability among non-aflatoxigenic A. flavus isolates that require greater attention in order to design molecular experiment to distinguish true aflatoxigenic from non-aflatoxigenic A. flavus strains.

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