High-glycemic index carbohydrate increases nuclear factor-κB activation in mononuclear cells of young, lean healthy subjects1-3

Scott Dickinson, Dale P. Hancock, Peter Petocz, Antonio Ceriello, Jennie Brand-Miller

Research output: Contribution to journalArticle

Abstract

Background: High-glycemic index diets have been linked to greater risk of cardiovascular disease and type 2 diabetes. Postprandial glycemia within the normal range may promote oxidative stress and inflammatory processes underlying the development of disease. Objective: We explored acute differences in the activation of the inflammatory marker nuclear factor-κB after consumption of 2 carbohydrate meals matched for macronutrient and micronutrient composition but differing in glycemic index. Design: After an overnight fast, 10 young, lean healthy subjects were fed in random order 3 meals providing 50 g of available carbohydrate as glucose, white bread, or pasta. Venous blood samples were collected at 0, 1, 2, and 3 h, and nuclear proteins were extracted from mononuclear cells. Changes in nuclear factor-κB-p65 proteins were detected by Western blotting. Acute changes in other markers of oxidative stress (nitrotyrosine and soluble intercellular adhesion molecule-1) were also assessed. Results: The maximum increase in nuclear factor-κBactivation was similar after the bread meal [mean (±SEM) area under the curve: 69 ± 16% optical density · h] and the glucose challenge (75 ± 9% optical density · h), but was 3 times higher than after the pasta meal (23 ± 5% optical density · h) (P <0.05). Similarly, changes in nitrotyrosine, but not soluble intercellular adhesion molecule-1, were higher after glucose and bread than after pasta (P=0.01 at 2 h). Conclusions: The findings suggest that high-normal physiologic increases in blood glucose after meals aggravate inflammatory processes in lean, young adults. This mechanism may help to explain relations between carbohydrates, glycemic index, and the risk of chronic disease.

Original languageEnglish
Pages (from-to)1188-1193
Number of pages6
JournalAmerican Journal of Clinical Nutrition
Volume87
Issue number5
Publication statusPublished - May 1 2008

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Glycemic Index
glycemic index
pasta
absorbance
Meals
Carbohydrates
carbohydrates
Bread
breads
blood glucose
glucose
adhesion
oxidative stress
white bread
Intercellular Adhesion Molecule-1
Glucose
nuclear proteins
cells
dietary minerals
young adults

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

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High-glycemic index carbohydrate increases nuclear factor-κB activation in mononuclear cells of young, lean healthy subjects1-3. / Dickinson, Scott; Hancock, Dale P.; Petocz, Peter; Ceriello, Antonio; Brand-Miller, Jennie.

In: American Journal of Clinical Nutrition, Vol. 87, No. 5, 01.05.2008, p. 1188-1193.

Research output: Contribution to journalArticle

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