High incidence of BK virus large-T-antigen-coding sequences in normal human tissues and tumors of different histotypes

M. De Mattei, F. Martini, A. Corallini, M. Gerosa, K. Scotlandi, P. Carinci, G. Barbanti-Brodano, M. Tognon

Research output: Contribution to journalArticle

Abstract

T-antigen (TAg) coding sequences specific for BK virus (BKV) were detected, by PCR amplification followed by Southern-blot hybridization, in a high percentage of human tumors and tumor-cell lines, as well as in normal tissues, by analysis of 189 specimens. Specifically, the BKV early region was detected in 85% of brain tumors and in all normal brain tissues, in 78% of osteosarcomas, in 38% of Swing's tumors, in 40% of normal bone specimens and in 71% of normal peripheral blood cell samples. Wilms' tumor tissues used as a control were all negative for BKV sequences. RT-PCR analysis indicated that TAg coding sequences were expressed in specimens carrying BKV early region, ranging from 64% of the osteosarcomas to 100% of glioblastomas, Ewing's tumors, peripheral blood cells and normal bone. Moreover, DNA sequencing performed in 12 different positive samples revealed that the amplified PCR products are identical to the early-region sequence of wild-type BKV. The role of BKV TAg and its possible mechanism of action in human tumorigenesis are discussed.

Original languageEnglish
Pages (from-to)756-760
Number of pages5
JournalInternational Journal of Cancer
Volume61
Issue number6
DOIs
Publication statusPublished - 1995

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BK Virus
Viral Tumor Antigens
Incidence
Neoplasms
Osteosarcoma
Polymerase Chain Reaction
Blood Cells
Bone and Bones
Ewing's Sarcoma
Wilms Tumor
Glioblastoma
Southern Blotting
Tumor Cell Line
DNA Sequence Analysis
Brain Neoplasms
Carcinogenesis
Brain

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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High incidence of BK virus large-T-antigen-coding sequences in normal human tissues and tumors of different histotypes. / De Mattei, M.; Martini, F.; Corallini, A.; Gerosa, M.; Scotlandi, K.; Carinci, P.; Barbanti-Brodano, G.; Tognon, M.

In: International Journal of Cancer, Vol. 61, No. 6, 1995, p. 756-760.

Research output: Contribution to journalArticle

De Mattei, M, Martini, F, Corallini, A, Gerosa, M, Scotlandi, K, Carinci, P, Barbanti-Brodano, G & Tognon, M 1995, 'High incidence of BK virus large-T-antigen-coding sequences in normal human tissues and tumors of different histotypes', International Journal of Cancer, vol. 61, no. 6, pp. 756-760. https://doi.org/10.1002/ijc.2910610603
De Mattei, M. ; Martini, F. ; Corallini, A. ; Gerosa, M. ; Scotlandi, K. ; Carinci, P. ; Barbanti-Brodano, G. ; Tognon, M. / High incidence of BK virus large-T-antigen-coding sequences in normal human tissues and tumors of different histotypes. In: International Journal of Cancer. 1995 ; Vol. 61, No. 6. pp. 756-760.
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