High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria

A. Mellmann; F. Bimet; C. Bizet; A. D. Borovskaya; R. R. Drake; U. Eigner; A. M. Fahr; Y. He; E. N. Ilina; M. Kostrzewa; T. Maier; L. Mancinelli; W. Moussaoui; G. Prévost; L. Putignani; C. L. Seachord; Y. W. Tang; D. Harmsen

doi:10.1128/JCM.00921-09

High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria

A. Mellmann, F. Bimet, C. Bizet, A. D. Borovskaya, R. R. Drake, U. Eigner, A. M. Fahr, Y. He, E. N. Ilina, M. Kostrzewa, T. Maier, L. Mancinelli, W. Moussaoui, G. Prévost, L. Putignani, C. L. Seachord, Y. W. Tang, D. Harmsen

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article › peer-review

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).

Original language	English
Pages (from-to)	3732-3734
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	11
DOIs	https://doi.org/10.1128/JCM.00921-09
Publication status	Published - Nov 2009

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.00921-09

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Mellmann, A., Bimet, F., Bizet, C., Borovskaya, A. D., Drake, R. R., Eigner, U., Fahr, A. M., He, Y., Ilina, E. N., Kostrzewa, M., Maier, T., Mancinelli, L., Moussaoui, W., Prévost, G., Putignani, L., Seachord, C. L., Tang, Y. W., & Harmsen, D. (2009). High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. Journal of Clinical Microbiology, 47(11), 3732-3734. https://doi.org/10.1128/JCM.00921-09

High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. / Mellmann, A.; Bimet, F.; Bizet, C.; Borovskaya, A. D.; Drake, R. R.; Eigner, U.; Fahr, A. M.; He, Y.; Ilina, E. N.; Kostrzewa, M.; Maier, T.; Mancinelli, L.; Moussaoui, W.; Prévost, G.; Putignani, L.; Seachord, C. L.; Tang, Y. W.; Harmsen, D.

In: Journal of Clinical Microbiology, Vol. 47, No. 11, 11.2009, p. 3732-3734.

Research output: Contribution to journal › Article › peer-review

Mellmann, A, Bimet, F, Bizet, C, Borovskaya, AD, Drake, RR, Eigner, U, Fahr, AM, He, Y, Ilina, EN, Kostrzewa, M, Maier, T, Mancinelli, L, Moussaoui, W, Prévost, G, Putignani, L, Seachord, CL, Tang, YW & Harmsen, D 2009, 'High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria', Journal of Clinical Microbiology, vol. 47, no. 11, pp. 3732-3734. https://doi.org/10.1128/JCM.00921-09

Mellmann, A. ; Bimet, F. ; Bizet, C. ; Borovskaya, A. D. ; Drake, R. R. ; Eigner, U. ; Fahr, A. M. ; He, Y. ; Ilina, E. N. ; Kostrzewa, M. ; Maier, T. ; Mancinelli, L. ; Moussaoui, W. ; Prévost, G. ; Putignani, L. ; Seachord, C. L. ; Tang, Y. W. ; Harmsen, D. / High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria. In: Journal of Clinical Microbiology. 2009 ; Vol. 47, No. 11. pp. 3732-3734.

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abstract = "Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).",

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AU - Borovskaya, A. D.

AU - Drake, R. R.

AU - Eigner, U.

AU - Fahr, A. M.

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AU - Ilina, E. N.

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AU - Maier, T.

AU - Mancinelli, L.

AU - Moussaoui, W.

AU - Prévost, G.

AU - Putignani, L.

AU - Seachord, C. L.

AU - Tang, Y. W.

AU - Harmsen, D.

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High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria

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