High level antibody response to retrovirus-associated but not to melanocyte lineage-specific antigens in mice protected against B16 melanoma

Lucia Sfondrini, Daniele Morelli, Alessandra Bodini, Maria I. Colnaghi, Sylvie Ménard, Andrea Balsari

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mice vaccinated with Mycobacterium tuberculosis Ag38 gene-transduced B16 melanoma cells showed significant protection from intravenous challenge with parental B16 melanoma cells. No cytotoxic T-cell activity was found against melanoma cells, although the endogenous presence of the mycobacterial gene induced a preferential Th1 response. After immunization, a low serological response against melanoma cells was detected, while a high titer of antibodies directed to parental B16 cells, mainly of IgG2(a) isotype, was found in protected mice after challenge. These antibodies exhibited complement-dependent cytotoxicity against melanoma cells in vitro, while in vivo, used in passive immunization, they induced a decrease in a number of experimental B16 lung metastases. Most of the antibodies were directed against endogenous murine leukemia viruses. No reactivity against melanocyte lineage-specific antigens was observed. In particular, no reactivity was found in sera from protected mice against tyrosinase-related protein 2 (TRP- 2), either stably expressed in a non-melanoma cell line or obtained by in vitro transcription-translation, or against tyrosinase, TRP-I and gp100 antigens immunoprecipitated from B16 cells. Thus, in the B16 murine model, the presence of dominant viral antigens induces a very strong humoral response that might be protective and may inhibit or mask the presence of minor clonotypes.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalInternational Journal of Cancer
Volume83
Issue number1
DOIs
Publication statusPublished - 1999

Fingerprint

Experimental Melanomas
Melanocytes
Retroviridae
Antibody Formation
Antigens
Melanoma
Antibodies
Murine Leukemia Viruses
Passive Immunization
Monophenol Monooxygenase
Viral Antigens
Masks
Mycobacterium tuberculosis
Genes
Immunization
Immunoglobulin G
Neoplasm Metastasis
T-Lymphocytes
Cell Line
Lung

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

High level antibody response to retrovirus-associated but not to melanocyte lineage-specific antigens in mice protected against B16 melanoma. / Sfondrini, Lucia; Morelli, Daniele; Bodini, Alessandra; Colnaghi, Maria I.; Ménard, Sylvie; Balsari, Andrea.

In: International Journal of Cancer, Vol. 83, No. 1, 1999, p. 107-112.

Research output: Contribution to journalArticle

Sfondrini, Lucia ; Morelli, Daniele ; Bodini, Alessandra ; Colnaghi, Maria I. ; Ménard, Sylvie ; Balsari, Andrea. / High level antibody response to retrovirus-associated but not to melanocyte lineage-specific antigens in mice protected against B16 melanoma. In: International Journal of Cancer. 1999 ; Vol. 83, No. 1. pp. 107-112.
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